Measurement uncertainty in clinical chemistry: ISO 20914 versus nordtest or intermediate precision versus bias.

Aim: Measuring uncertainty (MU) is crucial to ensure the accuracy and precision of laboratory results. This study compares the ISO 20914 and Nordtest guidelines to analyze the MU values for 20 clinical chemistry analytes over six months.

Methods: The researchers calculated MU components, including within-laboratory reproducibility (Rw), laboratory analytical performance bias ((bias)), and combined standard uncertainty (), based on internal quality control and external quality assessment data.

A comparative analysis of Sigma metrics using conventional and alternative formulas.

Background And Aim: The Six Sigma approach, employing Sigma Metrics (SM), is commonly used to evaluate analytical performance in clinical laboratories. However, there is ongoing debate regarding the suitability of the conventional SM formula, which incorporates total allowable error (TEa) and bias. To address this, an alternative formula based on within-subject biological variation (CV) as the tolerance range (TR) has been proposed.

Negligible Specimen Hemolysis Is Observed With Sarstedt S-Monovette Coagulation Tubes in Aspiration Mode.

Objectives: We aimed to compare the levels of hemolysis in the blood collected using the vacuum and aspiration modes via Sarstedt S-Monovette coagulation tubes.

Methods: Forty volunteers were included in the study. Blood samples were collected using two different modes in the S-Monovette citrate tube (Sarstedt AG).

Could S-Monovette Serum Gel Tubes be Used for Clinical Chemistry Analysis Instead of Serum Separator Tube II Advance?

Background: We aimed to compare the Sarstedt S-Monovette serum gel tube and the BD (Becton, Dickinson and Company) Serum Separator Tube II (SST II) Advance based on technical specifications and tests results.

Methods: One hundred and twenty volunteers were included in the technical evaluation and 42 of 120 volunteers in the clinical evaluation. Blood was collected into S-Monovette, and SST II.

Interferograms plotted with reference change value (RCV) may facilitate the management of hemolyzed samples.

Background: The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Preanalytical Phase (WG-PRE) have recommended an algorithm based on the reference change value (RCV) to evaluate hemolysis. We utilized this algorithm to analyze hemolysis-sensitive parameters.

Methods: Two tubes of blood were collected from each of the 10 participants, one of which was subjected to mechanical trauma while the other was centrifuged directly.

Evaluation of the Relationship Between Transcutaneous Bilirubin Measurement and Total Serum Bilirubin in Neonatal Patients Followed for Jaundice.

Objectives: Jaundice is a physiological condition caused by hyperbilirubinemia, which is common in neonatal period. However, severe hyperbilirubinemia can cause kernicterus, which is a serious condition that leads to neurological problems. In this study, we aimed to investigate whether it is safe to use transcutaneous bilirubin (TcB) instead of blood for the evaluation of jaundice by comparing TcB measurement with standard total serum bilirubin (TSB) measurement values.

Effect of haemolysis on an enzymatic measurement of ethanol.

Introduction: We investigated the interference of haemolysis on ethanol testing carried out with the Synchron assay kit using an AU680 autoanalyser (Beckman Coulter, Brea, USA).

Materials And Methods: Two tubes of plasma samples were collected from 20 volunteers. Mechanical haemolysis was performed in one tube, and no other intervention was performed in the other tube.