Phylogenetic studies on the genus (Psathyrellaceae, Basidiomycota) occurring in the bed of the Indus River, Punjab, Pakistan, reveal three new species.

During macrofungal surveys in 2019-2024, several specimens belonging to the family Psathyrellaceae were collected from the bed of the Indus River, Punjab, Pakistan. Phylogenetic analyses, based on ITS, LSU, and tef-1α sequences and morpho-anatomical study, confirmed the novelty and placement of three taxa in the genus . They are described as , , and .

Pseudomegasporoporia margallensis sp. nov. and Microporellus subadustus (Polyporaceae, Basidiomycota) newly described and recorded from Pakistan.

In this study, we describe Pseudomegasporoporia margallensis as a novel species and report Microporellus subadustus as a new record from Pakistan based on morphology and phylogeny. Both genera are firstly reported in the country. Pseudomegasporoporia margallensis sp.

Phytochemical Analysis and Antioxidant, Antimicrobial, and Antibiofilm Effects of a New Himalayan Lichen Usman and Khalid Growing in Pakistan.

Phytochemical composition and antimicrobial, antibiofilm, and antioxidant effects of a newly described Himalayan lichen Usman and Khalid growing in Pakistan were investigated. HPLC-DAD methods were used for identification of secondary metabolites in acetone and methanol extracts. The total phenolics content was measured using a spectrophotometric method.

Phenotypic and genotypic characterization of methicillin resistant associated with pyogenic infections.

Background And Objectives: Staphylococcal infections are one of the major infectious diseases affecting globally in spite of advances in development of antimicrobial agents. Knowledge and awareness about the local pattern and prevalence of MRSA infections plays a key role in treatment. The aim of this study was to identify MRSA strains by phenotypic and genotypic methods and to analyze the antibiotic susceptibility pattern of MRSA strains from patients attending a tertiary care hospital.

Exploring Species in Industrial Wastewater: Morphological and Molecular Insights from Isolates.

The genus holds economic significance due to its widespread distribution and diverse applications, including biological control, enzyme production, and various biotechnological uses. The accurate identification of species is crucial given their close association with human activities. Despite previous efforts in classification, a comprehensive analysis combining morphological and molecular approaches is necessary.

Phytochemical composition and antitumor activity of a new arctic lichen .

For the first-time, chemical composition and antitumor activity was investigated of a newly described lichen Usman & Khalid from the second highest plateau of the world (Deosai Plains, Pakistan). HPLC-UV method was used for identification of secondary metabolites and the acetone extract had higher values of TPC (41.90 mg GA/g and TFC (75.

Assessment of four dose calculation algorithms using IAEA-TECDOC-1583 with medium dependency correction factor (K) application.

Purpose: This study discusses the measurement of dose in clinical commissioning tests described in IAEA-TECDOC-1583. It explores the application of Monte Carlo (MC) modelled medium dependency correction factors (K) for accurate dose measurement in bone and lung materials using the CIRS phantom.

Methods: BEAMnrc codes simulate radiation sources and model radiation transport for 6 MV and 15 MV photon beams.

Individual- and Herd-Level Milk ELISA Test Status and Incidence for Paratuberculosis in Hubei Province, China.

subsp. (MAP) is responsible for the persistent infectious illness known as bovine paratuberculosis, which is one of the most easily overlooked diseases in China amid a lack of epidemiological data. In this study, we evaluated the agreement of milk and blood antibody tests for paratuberculosis and showed an overall agreement of 92.

The Identification of a Novel Nucleomodulin MbovP467 of and Its Potential Contribution in Pathogenesis.

is a causative agent of crucial diseases in both dairy and beef cattle leading to substantial economic losses. However, limited control measures for -related diseases exist due to a lack of understanding about the virulence factors of this pathogen, a common challenge in mycoplasma research. Consequently, this study aimed to characterize a novel nucleomodulin as a virulence-related factor of .

Two novel species of arctic-alpine lichen-forming fungi (Ascomycota, Megasporaceae) from the Deosai Plains, Pakistan.

Members of the lichen-forming fungal genus are known to occur in cold polar and high altitudinal environments. Two new species, and , are now described from the high altitude Deosai Plains, Pakistan, based on phenotypic, multigene phylogenetic and chemical evidence. Phenotypically, is characterised by orbicular light-brown thalli 1.

Acute and Subchronic Toxicity Assessment of Conventional Soxhlet Leaves Extracts in Sprague-Dawley Rats.

Background: In Ghana, leaves together with guava, pawpaw, and lime are processed into a decoction to treat fever. To encourage its usage, preclinical validation of the safety profile of the plant is required. The acute and subchronic toxicities of the conventional Soxhlet ethanolic leaves extract in Sprague-Dawley rats were investigated.

New species and new records of genus Buellia (lichenized Ascomycota, Caliciaceae) from Pakistan using electron microscopy and DNA barcoding techniques.

Three new species Buellia darelensis, B. densitheca, B. kashmirensis and two new records B.

First Report of Leaf Spot Caused by on in Pakistan.

Jasminum sambac L. is a species of jasmine native to a small region in the eastern Himalayas and is cultivated worldwide as an ornamental plant (USDA-ARS 2016). In Pakistan, it is cultivated for ornamental purposes throughout the country.

DNA barcoding and scanning electron microscopy reveals Erysiphe ahmadii sp. nov. and a new record Erysiphe populicola (Erysiphaceae, Helotiales) on salicaceous hosts from Pakistan.

A new species of powdery mildew fungus Erysiphe ahmadii and a new record, Erysiphe populicola, on Salicaceae are described from Pakistan. In addition to light microscopy, scanning electron microscopy is also done to clearly demonstrate the surface characters of chasmothecia. E.

Reagent Effects on the Activated Partial Thromboplastin Time Clot Waveform Analysis: A Multi-Centre Study.

(1) Background: The activated partial thromboplastin time (APTT)- based clot waveform analysis (CWA) quantitatively extends information obtained from the APTT waveform through its derivatives. However, pre-analytical variables including reagent effects on the CWA parameters are poorly understood and must be standardized as a potential diagnostic assay. (2) Methods: CWA was first analysed with patient samples to understand reagent lot variation in three common APTT reagents: Pathromtin SL, Actin FS, and Actin FSL.

A study on the genus Candolleomyces (Agaricales: Psathyrellaceae) from Punjab, Pakistan.

Many basidiomata of the genus Candolleomyces were found on sandy and loamy soil from the Indus Riverbed, Kot Addu District. A phylogenetic study was conducted to examine the occurrence of Candolleomyces sindhudeltae sp. nov.

Identification and taxonomic position of a new Pseudoomphalina species from Pakistan based on light, scanning electron microscopy, and molecular analysis.

Khanspur, a small town located near Ayubia National Park, is a famous mountainous resort lying in Galyat area of Khyber Pakhtunkhwa. It is included in one of the richest biodiversity hotspots in the country. Despite of extensive previous studies, many new species are awaiting to be documented, including macrofungi.

Comparison of four commercial dose calculation algorithms in different evaluation tests.

Background: Accurate and fast dose calculation is crucial in modern radiation therapy. Four dose calculation algorithms (AAA, AXB, CCC, and MC) are available in Varian Eclipse and RaySearch Laboratories RayStation Treatment Planning Systems (TPSs).

Objectives: This study aims to evaluate and compare dosimetric accuracy of the four dose calculation algorithms applying to homogeneous and heterogeneous media, VMAT plans (based on AAPM TG-119 test cases), and the surface and buildup regions.

Laccaria murina and L. pumila from Pakistan, a study based on SEM and nrITS molecular marker.

Two species of the genus Laccaria, are described as new reports for Pakistan. Laccaria murina has been collected from a Himalayan moist temperate forest in Khanspur, KP, while L. pumila was found in the moist temperate forests of Kumrat Valley in Dir Upper, KP, and at higher altitudes of the Deosai plains of Gilgit-Baltistan, Pakistan.

Two new species of genus (Agaricaceae, Agaricales) from Pakistan.

The genus of basidiomycetous fungi, , occurs worldwide, from subtropical to boreal latitudes. Several collections of were made during mycological field trips conducted in different forests of Margalla, Pakistan. An integrative framework combining morphological and phylogenetic data was employed for their study.