Automated Dynamic Inlet Microfluidics (ADIM) system: cost-effective biaxial nanoliter droplet on demand generation platform and its application in agglutination assays.

The paper demonstrates an adaptation of a Prusa Mini+ 3D printer through the integration of 3D printed modules, creating a system capable of producing varied droplets from multiple Eppendorf tubes. Building upon our previous model, this system enhances calibration methodology enabling any fused deposition modeling (FDM) printer to produce mono-disperse droplets (coefficient of variance (CV%) <2% for train of 100 droplets) with 6900 assays per hour rate. The cost of the developed system is 85% lower than that of existing droplet generation solutions on the market, and 30% more economical than the previous iteration of the system.

Automated dynamic inlet microfluidics system: 3D printer adaptation for cost-effective, low volume, on-demand multi-analyte droplet generator.

The paper demonstrates an adaptation of a 3D printer (Prusa Mini+) with novel modules to develop a droplet generation system that generates combinatorial droplets from a standard 96 well plate. The calibration methodology developed would allow any fused deposition modeling (FDM) printer to generate monodisperse droplets (coefficient of variance (CV%) < 5%) from well plates or vials of any geometry. The system maintains precision across various volumes while maintaining a C.