Inhibition of experimental angiogenesis of cornea by various doses of doxycycline and combination of triamcinolone acetonide with low-molecular-weight heparin and doxycycline.

Purpose: To evaluate the effect of topically administered doxycycline in various doses; the combination of triamcinolone acetonide and low-molecular-weight heparin (LMWH); and the combination of triamcinolone acetonide and doxycycline on experimental corneal neovascularization in rats.

Methods: This project is the combination of 2 separate studies. First, the chemical cauterization of corneas in 36 eyes of 36 Long Evans male rats was performed by using silver nitrate/potassium nitrate sticks.

Threshold parameters for choroidal vessel closure with the photosensitizer, lambda 27.

Objective: To determine the threshold photodynamic therapy parameters, required for choroidal vessel closure, with the photosensitizer, Lambda 27, in Dutch belted rabbits, using fluorescein angiography and histopathology.

Design: A pre-clinical experiment.

Place And Duration Of Study: Department of Ophthalmology at the Tulane University Health Sciences Center, between June 2001 - July 2002.

The effect of intravitreally injected povidone-iodine on Staphylococcus epidermidis in rabbit eyes.

Purpose: Firstly, the aim of this study was to determine the maximally tolerated dose of intravitreally injected povidone-iodine (PVP-I). A second aim was to test the efficacy of PVP-I on rabbit eyes infected intravitreally with Staphylococcus epidermidis.

Methods: Phase I of the study used 16 New Zealand albino rabbits, divided into 4 groups (n=4 each).

Intravitreal toxicity of high-dose etanercept.

Purpose: The aim of this study was to evaluate the retinal toxicity of high-dose intravitreal etanercept, a U.S. Food and Drug Administration-approved anti-inflammatory drug, in the rabbit model.

Prevention of corneal neovascularization: evaluation of various commercially available compounds in an experimental rat model.

Purpose: To evaluate the effect of topically administered agents doxycycline, triamcinolone, low molecular weight heparin (LMWH), and flurbiprofen on experimental corneal neovascularization in rats.

Methods: Chemical cauterization of cornea was performed in 36 eyes of 36 rats by using silver nitrate/potassium nitrate sticks. Topical instillation of doxycycline, triamcinolone, LMWH, flurbiprofen, and normal saline solution was continued for 7 days.

The effect of combinations of flurbiprofen, low molecular weight heparin, and doxycycline on the inhibition of corneal neovascularization.

Purpose: To determine the efficacy of various combinations of flurbiprofen, low molecular weight heparin (LMWH), and doxycycline on the inhibition of corneal neovascularization in rats.

Methods: Chemical cauterization of the cornea in 32 eyes of 32 rats was performed using silver nitrate/potassium nitrate sticks. The animals were divided into 4 groups.

Retinal toxicity of intravitreal 5-fluorouracil in silicone-filled rabbit eyes.

Objective: To determine the retinal toxicity of 5-fluorouracil, by means of electroretinography and histopathology, at different doses in vitrectomized rabbit eyes injected with silicone oil.

Design: A pre-clinical experimental study.

Place And Duration Of Study: The Louisiana State University Eye Center and the Department of Ophthalmology at the Tulane University Health Sciences Center, between June 1999 and May 2002.

Intravitreal toxicity of levofloxacin and gatifloxacin.

Background And Objective: To assess the retinal toxicity of varying concentrations of intravitreally injected gatifloxacin and levofloxacin.

Materials And Methods: In this experimental, controlled study, levofloxacin (initial concentration = 25 mg/mL) and gatifloxacin (initial concentration = 2 mg/mL) were titrated using 5% dextrose solution to concentrations of 2,500 to 156 microg/0.1 mL and 400 to 50 microg/0.

Intravitreal toxicity of moxifloxacin.

Purpose: To assess the retinal toxicity of various concentrations of intravitreally administered moxifloxacin, a fourth-generation fluoroquinolone.

Methods: Ten New Zealand albino rabbits were divided into five groups. The initial concentration of moxifloxacin (400 mg/250 mL) was titrated using 5% dextrose solution to concentrations (320 microg/0.

Intravitreal toxicity of garenoxacin.

Purpose: To assess the retinal toxicity of varying concentrations of intravitreally injected garenoxacin.

Methods: Twenty eyes of 20 New Zealand albino rabbits were used for this study. The animals were anesthetized with ketamine (35-50 mg/kg) and xylazine (3-5 mg/kg).

Effect of squalamine on iris neovascularization in monkeys.

Purpose: To investigate the effect of squalamine, an antiangiogenic aminosterol, in an experimental model of iris neovascularization.

Methods: Iris neovascularization was created in cynomolgus monkeys by occluding retinal veins with an argon laser and inducing persistent hypotony with a central corneal suture. Twenty-four eyes were treated in three groups.