Rapid detection of Brucella cells using a gold nanoparticle-based aptasensor via a simple colorimetric method.

Background: Brucellosis is a major worldwide zoonotic disease that is caused by Brucella spp. and threatens the health of communities. Novel methods for rapid detection of Brucella bacteria are beneficial and necessary in preventing infection and subsequent economic losses.

In vitro evaluation of two novel Escherichia bacteriophages against multiple drug resistant avian pathogenic Escherichia coli.

Background: In recent years, there has been a growing interest in phage therapy as an effective therapeutic tool against colibacillosis caused by avian pathogenic Escherichia coli (APEC) which resulted from the increasing number of multidrug resistant (MDR) APEC strains.

Methods: In the present study, we reported the characterization of a new lytic bacteriophage (Escherichia phage AG- MK-2022. Basu) isolated from poultry slaughterhouse wastewater.

Synthesis and Application of Task-Specific Bimetal-Organic Frameworks in the Synthesis of Biological Active Spiro-Oxindoles.

The use of click chemistry as a smart and suitable method for the development of new heterogeneous catalysts is based on metal-organic frameworks as well as the production of organic compounds. The development of the click chemistry method can provide a new strategy to achieve superior properties of MOFs. Here, the two metals Co and Fe are used to create a bimetallic-organic framework.

PCR Development for Analysis of Some Type II Toxin-Antitoxin Systems, relJK, mazEF3, and vapBC3 Genes, in Mycobacterium tuberculosis and Mycobacterium bovis.

Toxin-Antitoxin (TA) systems are some small genetic modules in bacteria that play significant roles in resistance and tolerance development to antibiotics. Whole genome sequencing (WGS) is an effective method to analyze TA systems in pathogenic Mycobacteria. However, this study aimed to use a simple and inexpensive PCR-Sequencing approach to investigate the type II TA system.

Antimicrobial susceptibility, virulence genes and genomic characterization of Trueperella pyogenes isolated from abscesses in dairy cattle.

Trueperella pyogenes is an opportunistic animal pathogen mainly associated with various suppurative infections in wild and domestic animals. Limited studies have investigated the pathogenesis of diseases caused by this pathogen. The main objective of the current study was to investigate the prevalence, phenotypic properties, virulence genotypes, antimicrobial susceptibility and genetic characterization of T.

Advanced nano biosensors for rapid detection of zoonotic bacteria.

An infectious disease that is transmitted from animals to humans and vice-versa is called zoonosis. Bacterial zoonotic diseases can re-emerge after they have been eradicated or controlled and are among the world's major health problems which inflict tremendous burden on healthcare systems. The first step to encounter such illnesses can be early and precise detection of bacterial pathogens to further prevent the following losses due to their infections.

Evaluation of Mutations Related to Streptomycin Resistance in Mycobacterium tuberculosis Clinical Isolates.

Drug resistance to streptomycin in the clinical isolates of Mycobacterium tuberculosis (MTB) needs special consideration. It can mostly be caused by mutations in four genes with the names rpsL, rrs, gidB, and whiB7. The main objective of this study was the evaluation of the type and frequency of mutations in these mentioned genes using the PCR-sequencing method.

Expression of virulence factor genes in co-infections with Trueperella pyogenes isolates and other bacterial pathogens; an in vivo study.

Trueperella pyogenes is an opportunistic bacterial pathogen causing several infectious diseases, including metritis, mastitis and abscesses in domestic animals such as dairy cattle. Several virulence proteins are released by T. pyogenes strains contributing to the pathogenic and causing disease potential of this pathogen.

High Prevalence of Multidrug Resistance and Biofilm-Formation Ability Among Avian Isolated from Broilers in Iran.

The present study was conducted to determine the antimicrobial resistance pattern and biofilm-formation ability in 100 Avian-Pathogenic (APEC) isolated from colibacillosis-suspected broilers and 100 Avian Fecal (AFEC) isolates from healthy broilers in Hamedan, Iran. All isolates were screened by polymerase chain reaction for antimicrobial resistance genes, class 1 and 2 integrons, and biofilm-associated genes. Besides, we assessed the possible relationship between biofilm-formation ability antibiotic resistance patterns, genetic background, and the pathogenicity of APEC strains.

Complete genome sequence of Trueperella pyogenes strain Arash114, isolated from the uterus of a water buffalo (Bubalus bubalis) in Iran.

Objective: Trueperella pyogenes has been considered a major causative agent of metritis, abortion, and death in a broad range of domestic and wild animals, including cattle, swine, sheep, goats, camels, buffalo, deer, antelopes, reptiles, and birds.

Data Description: Here, we report the complete chromosome sequence of Trueperella pyogenes strain Arash114, isolated from the uterus of a water buffalo (Bubalus bubalis) died due to the infection caused by this pathogen. The genome assembly comprised 2,338,282 bp, with a 59.

L. Essential Oil: A Promising Antibacterial and Antivirulence Agent Against Multidrug-Resistant .

L. (cumin) is valued for its aromatic and medicinal properties. There are several reports of antibacterial activity of essential oil (CcEO).

Investigation of antimicrobial susceptibility and virulence factor genes in isolated from clinical mastitis cases of dairy cows.

is an opportunistic pathogen causing important diseases including mastitis and metritis in domestic animals such as dairy cows leading to prominent economic losses in food production industry. The aim of this study was to investigate bacterial species, antimicrobial susceptibility, and presence of virulence factor genes and genotyping of .  isolates associated with summer mastitis cases from 22 different farms around Tehran, Iran.

Siderophores: Importance in bacterial pathogenesis and applications in medicine and industry.

Iron is an essential element for all microorganisms. Siderophores are low-weight, high-affinity iron chelating molecules produced in response to iron deficiency by Gram-positive and Gram-negative bacteria which also known as essential virulence factors of bacteria. Several studies have indicated that defective production and/or function of these molecules as well as iron acquisition systems in pathogens are associated with a reduction in pathogenicity of bacteria.

Serogrouping, phylotyping, and virulence genotyping of commensal and avian pathogenic Escherichia coli isolated from broilers in Hamedan, Iran.

In the present study, 100 Avian-Pathogenic Escherichia coli (APEC) isolates from colibacillosis-suspected broilers and 100 Avian Faecal Escherichia coli (AFEC) isolates from healthy broilers in Iran were examined by PCR for confirmation of their serogroups and phylogenetic background, and their association with ten virulence-associated genes (VAG) including fimC, iutA, chuA, sitA, iss, cvaA/B, hylA, stx1, stx2, and yjaA. Serogroups O78, O1, O2 and O18 were the prominent strains including 54 % of the APEC and 23 % of the AFEC strains. At phylotyping, the majority of APEC strains belonged to phylogenetic group E (22 %) while for the AFEC strains, half of the isolates were not assigned to any group but the predominant phylogroup was E (27 %).

Genomic characterisation, detection of genes encoding virulence factors and evaluation of antibiotic resistance of Trueperella pyogenes isolated from cattle with clinical metritis.

Trueperella pyogenes is one of the most important microorganisms causing metritis in post-partum cattle. Co-infection with other bacterial species such as Escherichia coli or Fusobacterium necrofurom increases the severity of the disease and the persistence of bacteria in utero. The aim of this study was to investigate the frequency of T.

Streptococcus pneumoniae quorum sensing and biofilm formation are affected by Thymus daenensis, Satureja hortensis, and Origanum vulgare essential oils.

The aim of this study was to investigate the effect of Thymus daenensis L., Satureja hortensis L., and Origanum vulgare L.

Molecular typing of of different origins based on the polymorphism of the gene: characterization of a novel type.

The present study was conducted to determine the molecular diversity of strains isolated from human, bovine and food samples based on the polymorphism of the gene. A total of 208 isolated from human, bovine raw milk and food samples were assessed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single locus sequence typing (SLST) methods, followed by determination of types using Ridom SpaServer. Altogether, 15 distinct RFLP patterns were recorded (I-XV).

Should we care about transmission of infectious bacterial agents through serum handling?

Immunological assays are valuable diagnostic tools to detect infections due to most of bacterial microorganisms. However, the question is how much safe are the common serum samples used as sources of antibodies in these assays? Here, we experimentally followed the issue. 10-fold serial dilutions of two Gram-positive, Staphylococcus aureus and Bacillus cereus, and one Gram-negative bacteria, Escherichia coli, were prepared and spiked into freshly taken blood samples of human and four animal species including sheep, goat, cattle, and horse.

Seroprevalence of Neospora caninum in slaughtered native cattle in Kurdistan province, Iran.

Neospora caninum is a worldwide distributed pathogen which causes abortion in cattle leading to economic loss in the cattle industry. The aim of this study was to determine the seroprevalence of N. caninum antibodies in the native cattle slaughtered in various areas of Kurdistan province (western Iran) from September 2010 to September 2011.