Gill alterations of whiteleg shrimp (Litopenaeus vannamei) exposed to cadmium under different experimental salinities.

Background: Heavy metals are one of the most important environmental pollutants in marine coastal ecosystems. Cadmium is a heavy metal that enters to marine environments via industrial wastes and oil production activities.

Objectives: This study were done to determine the toxicity of cadmium to Litopenaeus vannamei and to evaluate the histological changes in gill tissues after exposure to sublethal concentrations of cadmium at different salinities.

Impacts of sublethal concentrations of 17 α-ethinylestradiol (EE2) on growth, reproductive performance, and survival in red cherry shrimp Neocaridina davidi (Crustacea, Atyidae) during consecutive spawnings.

This study was conducted for the first time to investigate the long-term impacts of sublethal concentrations of 17 α-ethinylestradiol (EE2) on growth, survival, and reproductive performances in a model shrimp, the red cherry (Neocaridina davidi), females during five successive spawning steps for 7.5 months. Females were distributed in eighteen aquariums and continuously exposed to EE2 at six nominal concentrations of 0 (control), 0.

Immunotoxic effects of metal-based nanoparticles in fish and bivalves.

There is a global research interest in metal nanoparticles (MNPs) due to their diverse applications, rapidly increasing use, and increased presence in the aquatic environment. Currently, most MNPs in the environment are at levels unlikely to cause overt toxicity. Sub-lethal effects that MNPs may induce, notable immunotoxicity, could however have significant health implications.

The effect of nonylphenol exposure on the stimulation of melanomacrophage centers, estrogen and testosterone level, and ERα gene expression in goldfish.

The present study tried to measure the formation of melanomacrophage centers (MMCs) in various organs of male and female goldfish exposed to nonylphenol (NP) and aimed to assess its relationship with the main sexual hormones, estrogen receptor expression, and the pigment content of the MMCs. Immature goldfish were exposed to 10 and 10 M NP for 25 days. After obtaining blood for measuring testosterone and estrogen (E2) levels, tissue samples were collected from various organs for histological studies, quantifying pigments using ImageJ software and chemical analysis, and measuring ERα gene expression.

Selective induced apoptosis and cell cycle arrest in MCF7 and LNCap cell lines by skin mucus from round goby (Neogobius melanostomus) and common carp (Cyprinus carpio) through P53 expression.

The skin mucus in lower vertebrates such as fish with strong innate immune system has many unique and valuable bioactive compounds that can be used for inducing apoptosis in cancer cells. This study was looking for the cytotoxic potential of mucus from the two fish species, including round goby (Neogobius melanostomus) and common carp (Cyprinus carpio), and inducing apoptosis in MCF7 and LNCaP cancer cell lines via influencing P53 gene expression and cell cycle arrest. Results showed that the both mucus types have cytotoxic effects on the both cancer cell lines whereas they have no severe effect on normal primary fibroblast cells.

Using ovarian and brain cell culture from the Mullet, Liza klunzingeri, to assess the inhibitory effects of benzo[a]pyrene on aromatase activity.

We assessed the toxic effects of benzo[a]pyrene (BaP) on cell viability, aromatase (Aro) activity and steroid production using ovarian and brain cell cultures obtained from Mullet, Liza klunzingeri. The brain and ovary were minced and digested, and the cells were suspended in Leibovitz's L-15 medium supplemented with 15% and 20% fetal bovine serum. The cell suspensions were seeded on 25-cm cell-culture flasks at 1 × 10 cells/mL and incubated at 25 °C for 2 weeks.

Exposure of liver cell culture from the orange-spotted grouper, Epinephelus coioides, to benzo[a]pyrene and light results in oxidative damage as measured by antioxidant enzymes.

Among the various toxicants discharged into aquatic environments, benzo (a) pyrene (BaP) has been shown to effect on the antioxidant system of fish and the evaluation of its impact on biota is of considerable concern. The aim of the present study was to use the primary hepatocyte culture obtained from the orange-spotted grouper, Epinephelus coioides, to evaluate the adverse effects of benzo (a) pyrene (BaP) on cell viability and liver antioxidant system. BaP was selected for its high ability to produce reactive oxygen species (ROS) and oxidative stress.

Immunotoxicity of estrogen and nonylphenol on apoptosis and expression of ERs in goldfish macrophage: Opening new avenue for discovering the role of experimental model systems and sexes.

The expression of estrogen receptors (ERs) and their roles in important cell processes such as apoptosis in the macrophages exposed to estrogen/xenoestrogen have remained a complex secret. This study focused on the expression of estrogen receptors (ERs) and the stimulation of apoptosis in the macrophages from the two sexes of goldfish (Carassius auratus) exposed to 17-βestradiol (E2) and nonylphenol (NP) under in vivo and in vitro conditions. For the in vivo experiment, fish were exposed to NP (10 M and 10 M) and E2 (10 M) for 24 days.

Spontaneously contracting cell aggregates derived from grass carp heart as a promising tool in in vitro heart research.

Regarding challenges in isolation and maintenance of cultured heart cells, introduction of new in vitro heart model that is stable and exhibits long-term spontaneously contracting cell aggregates (SCCs), whose electrophysiological properties are comparable to the human heart, is required. This research aimed to establish cardiac primary cells and to evaluate the effects of culture conditions. Also the effect of fish age on beating SCC and cardiac cell morphology were studied.

Interruption of immune responses in primary macrophages exposed to nonylphenol provides insights into the role of ER and NF-KB in immunotoxicity of Persian sturgeon.

The severe decline in population of sturgeons due to pollution highlighted poor understanding about the immunotoxicological responses of sturgeons. This study was designed in three experiments to find out how nonylphenol (NP) interrupts some pro-inflammatory immune parameters in macrophages from Persian sturgeon (Acipencer persicous) as the oldest vertebrate model conserving intact innate immune system. After determination of IC values of NP (200 μM), some pro-inflammatory immune parameters and induced apoptosis in macrophages at low dose (10 nM) and high dose (100 nM) of NP and of 17β estradiol (E2) (positive control) were determined after 6, 24 and 48 h of the exposure (as the first experiment).

Optimization of macrophage isolation from the Persian sturgeon and the Caspian kutum fish: a comparative study.

The aim of this research was a comparative study on the isolation and culture of head kidney macrophages derived from Acipenser persicous and Rutilus frisii kutum as teleost and chondrostei species of fish. The macrophages were isolated by density gradient sedimentation, followed by adherence to a plastic surface. They exhibited strong phagocytic activity against bacteria.

Effects of the environmental endocrine disrupting compound benzo[a]pyrene on thyroidal status of abu mullet () during short-term exposure.

Benzo[a]Pyrene (BaP) is a ubiquitous polycyclic aromatic hydrocarbon (PAH) that has been shown to disrupt the metabolism of thyroid hormone. Then, the present investigation aimed to study the effects of BaP on thyroid function in . Fish were injected with 2, 10 and 25 mg/kg-bw of BaP.

CYP1A gene expression as a basic factor for fipronil toxicity in Caspian kutum fish.

The aim of this study was to assess the effects of fipronil insecticide on the Caspian kutum fish at different levels of biological organizations and to find possible relationship between these biomarkers. Different doses of fipronil (65, 130 and 200 mg/kg) were intraperitoneally administered to the fish for 2 weeks. After 7 and 14 days of exposure, alterations in organ-somatic index, tissue and DNA structure, oxidative stress and CYP1A gene expression in gill, liver, brain and kidney were studied.

Using cell apoptosis, micronuclei and immune alternations as biomarkers of phenanthrene exposure in yellowfin seabream (Acanthopagrus latus).

In the present study, the apoptosis and tissue changes in the spleen, as well as humoral immune-related parameters, micronuclei (MN) induction in blood cells and Ethoxyresorufin-O-deethylase (EROD) activity were investigated in yellowfin seabream (Acanthopagrus latus) after short-term exposure to phenanthrene (Phe). The fish were intraperitoneally injected with different concentrations (2, 20 and 40 mg kg) of Phe and tissue and blood samples were collected 1, 4, 7 and 14 days after injection. The concentrations of Phe in the fish liver increased 4 days after the experiment.

Comparison of waterborne and intraperitoneal exposure to fipronil in the Caspian white fish ( on acute toxicity and histopathology.

Fipronil is an effective insecticide widely used in agriculture with potential ecotoxicological consequences. The median lethal dose (LD) and concentration (LC) of fipronil in 16.3 g Caspian white fish, fingerlings were determined.

Using a liver cell culture from Epinephelus coioides as a model to evaluate the nonylphenol-induced oxidative stress.

The present study aimed to use primary liver cell culture derived from the orange-spotted grouper, Epinephelus coioides, to assess the toxic effects of nonylphenol (NP) on the hepatocyte viability and the liver antioxidant system. E. coioides was selected due to its commercial importance.

Assessment of immune status of yellowfin seabream (Acanthopagrus latus) during short term exposure to phenanthrene.

The aim of the present investigation was to assess the immune status in yellowfin seabream (Acanthopagrus latus) exposed to different concentrations of phenanthrene (Phe) for 14days. In addition, the Phe accumulation in the fish muscle was measured during the experiment. Fish were injected with different concentrations (0, 2, 20 and 40mg/kg) of Phe and samples were taken from tissue and blood of fish 1, 4, 7 and 14days after injection.

Environmentally induced tissue responses of hematopoietic system in abu mullet (Liza abu) and tiger tooth croaker (Otolithes ruber) from the Persian Gulf.

The present investigation aimed to assess the possibility of using plasma levels of erythropoietin (EPO) hormone and tissue changes of hematopoietic organs as biomarkers of environmental pollution in abu mullet (Liza abu) and tiger tooth croaker (Otolithes ruber) collected from Musa Creek (northwest of the Persian Gulf). 120 L. abu and O.

Evaluation of lymphoid tissue structure in Sole (Euryglossa orientalis) and Yellowfin Seabream (Acanthopagus latus) affected by environmental contaminants in the Persian Gulf.

This study sought to analyze structures of lymphatic tissues in two commercial fish species, e.g. Sole (Euryglossa orientalis) and Yellowfin Seabream (Acanthopagus latus), collected from five stations with varying levels of pollution in the Musa Creek near the Persian Gulf, e.

Using immune responses in Euryglossa orientalis and Acanthopagrus latus from Persian Gulf as indicators of environmental health.

One hundred and twenty sole (Euryglossa orientalis) and yellowfin seabream (Acanthopagrus latus) fishes were captured from five stations at the Musa Creek (northwest of the Persian Gulf): petrochemical, Gaffari, Majidieh, Ghazaleh, and Zangi stations. Blood was collected from the caudal vein as samples. Tissue samples were also taken from the spleen and head kidney, and tissue sections were prepared according to routine histological methods.

Application of biomarkers in Epaulet grouper (Epinephelus stoliczkae) to assess chromium pollution in the Chabahar Bay and Gulf of Oman.

In the present study plasma levels of erythropoietin (EPO) hormone and kidney histological changes were assessed as biomarkers to evaluate the chromium pollution in Epaulet grouper (Epinephelus stoliczkae) obtained from different areas in Chabahar Bay and Gulf of Oman. Chemical analyses included the evaluation of chromium in the fish kidney tissue and sediment. The highest and lowest concentrations of chromium were measured in the kidney of fish and sediment collected from Konarak (47.

Heavy metals in selected tissues and histopathological changes in liver and kidney of common moorhen (Gallinula chloropus) from Anzali Wetland, the south Caspian Sea, Iran.

The present study aimed to measure the concentrations of Sn, Pb, Zn, Hg, Cu, Ni and Cd in the muscle and liver of 40 Common Moorhens (Gallinula chloropus) hunted from four stations in Anzali Wetland (Pirbazar, Ghalam-Koudeh, Selkeh and Abkenar). The histopathologic alteration index (HAI) of liver and kidney was also assessed in these birds. The highest concentrations of selected metals were measured in the liver of birds collected from Ghalam-Koudeh (Pb: 4.

Detection of benzo[a]pyrene-induced immunotoxicity in orange spotted grouper (Epinephelus coioides).

This study aimed to investigate the effects of benzo[a]pyrene (BaP) on immune status of orange spotted grouper (Epinephelus coioides). Fish were injected with 2, 20 and 35 mg/kg-bw of BaP and were kept under laboratory conditions for 14 days. Blood samples were taken at days 1, 4, 7, and 14 and changes in total WBC and RBC, phagocytosis, lysozyme activity, lysosomal membrane stability, immunoglobulin M (IgM) level and antibacterial activity were evaluated.

An assessment of hematological and biochemical responses in the tropical fish Epinephelus stoliczkae of Chabahar Bay and Gulf of Oman under chromium exposure: ecological and experimental tests.

The aim of this investigation was to evaluate the effect of chromium on hematological and biochemical parameters in Epaulet Grouper, Epinephelus stoliczkae of Chabahar Bay and Gulf of Oman by ecological and experimental tests. Spatial evaluation of ecological test results showed these parameters had significant difference among some sampling sites. Examination of hematological and biochemical profiles on Epaulet Grouper was performed after 0.