Optical characterization of arterial apoptosis.

Apoptosis is a biological hallmark of both acute and chronic vascular pathology. It contributes to erosion and rupturing of atherosclerotic plaques, causing stroke and myocardial infarction, and plays an important role in post-angioplastic remodeling. Therefore, apoptosis is intensively studied in both explanatory and interventional vascular studies.

cNGR: a novel homing sequence for CD13/APN targeted molecular imaging of murine cardiac angiogenesis in vivo.

Objective: Previously, the peptide sequence cNGR has been shown to home specifically to CD13/APN (aminopeptidase N) on tumor endothelium. Here, we investigated the feasibility of selective imaging of cardiac angiogenesis using the cNGR-CD13/APN system.

Methods And Results: CD13/APN induction and cNGR homing were studied in the murine myocardial infarction (MI) model.

Past, present, and future of annexin A5: from protein discovery to clinical applications.

In this article, we review the clinical aspects of imaging with the programmed cell-detecting protein annexin A5 (anxA5). AnxA5 binds to phosphatidylserine, which is one of the "eat me" signals at the surface of the apoptotic cell. This biologic property forms the basis for the development of anxA5 as a diagnostic tool.

Annexin A5 down-regulates surface expression of tissue factor: a novel mechanism of regulating the membrane receptor repertoir.

Phosphatidylserine (PtdSer) is exposed on the external leaflet of the plasma membrane during apoptosis. The protein annexin A5 (anxA5) shows high affinity for PtdSer. When anxA5 binds to the PtdSer-expressing membranes during apoptosis, it crystallizes as an extended two-dimensional network and activates thereby a novel portal of cell entry that results in the internalization of the PtdSer-expressing membrane patches.