Cryopreservation of Pig Semen Using a Quercetin-Supplemented Freezing Extender.

Reactive oxygen species (ROS) produced during freeze−thaw procedures cause oxidative damage to the sperm, reducing fertility. We aimed to improve the post-thaw quality of pig sperm by quercetin (QRN) supplementation to reduce the cryodamage associated with the freeze−thaw procedure. Four equal aliquots of pooled boar semen were diluted with a freezing extender supplemented with different concentrations of QRN (0, 25, 50, and 100 µM) and then were subjected to cryopreservation in liquid nitrogen.

Quercetin improves the apoptotic index and oxidative stress in post-thaw dog sperm.

Freeze storage of ejaculated sperms is a crucial technique for the semen preservation of valuable pet animals such as dogs. The current study was conducted to investigate if quercetin (QRN) may ameliorate apoptosis and oxidative stress in post-thaw dog sperm. Herein, we evaluated the post-thaw apoptosis and oxidative stress after treatment with QRN (control, 25, 50, and 100 μM) in the freezing of dog semen.

Use of polyvinyl alcohol as a chemically defined compound in egg yolk-free extender for dog sperm cryopreservation.

The objectives of this study were to investigate the effects of polyvinyl alcohol (PVA) as a chemically defined compound in egg yolk (EY)-free extender by determining the appropriate concentration of PVA and the effect of pH adjustment in EY-free PVA extenders on dog spermatozoa. Spermatozoa (1 × 10  cells/ml) were frozen with EY-free extenders supplemented with 0 (control), 0.025, 0.