Impact of the corpus luteum and follicular parameters on the levels of plasma progesterone and the prediction of pregnancy in Holstein dairy cows.

The objective of this study was to investigate the reliability of corpus luteum and dominant follicles, imaging parameters on the plasma progesterone, and prediction of pregnancy in cows. In total, 48 cows were used in this study and underwent Ovsynch program. Ultrasound imaging of the ovaries was done at the time of PG shots.

Expression of genes for transport of water and angiogenesis, as well as biochemical biomarkers in Holstein dairy cows during the ovsynch program.

Changes in expression of genes associated with angiogenesis and transport of water by cells, as well as biomarkers of oxidative stress were determined at specific times during the ovsynch protocol to synchronize estrus and breed Holstein dairy cows. Blood samples were taken from 82 lactating Holstein cows at the time of the 1st GnRH injection (G1), 7 days later at the time of the PGF2a (PG) injection, and 48 h after the PGF2a treatment when the second injection of GnRH was administered (G2). The serum was analyzed for malondialdehyde (MDA), reduced glutathione (GSH), glutathione peroxidase (GPX), nitric oxide (NO), catalase (CAT), and total antioxidant capacity (TAC).

The vaginal and uterine blood flow changes during the ovsynch program and its impact on the pregnancy rates in Holstein dairy cows.

Aim: OvSynch is a hormonal protocol for synchronization of estrus and use of artificial insemination (AI) at an optimal time without adverse effects on the ovaries or uterus. This study investigated the use of noninvasive color Doppler ultrasound to assess changes in uterine and vaginal blood flow during the Ovsynch program for synchronization of estrus and its relation to the pregnancy rates in Holstein cows.

Materials And Methods: The experimental cows received an intramuscular dose of 10 μg of a GnRH analogue (G1), followed 7 days later with an intramuscular injection of synthetic prostaglandin F2α (P: PGF2α) analogue (500 μg cloprostenol sodium), and given a 10 μg, injection of the GnRH analogue (G2) i.

Some studies on the morphological aspects of buffalo oocytes in relation to the ovarian morphology and culture condition.

The present study was conducted to establish the effects of ovarian morphology on oocyte quantity and quality, as well as the effect of preincubated granulosa cells (PGCs) on in vitro maturation of buffalo oocytes and steroid hormones production. A total of 52 ovarian pairs were grouped into three types: type I (with functional corpus luteum), type II (with regressed corpus luteum), and type III (without corpus luteum). The number of follicles and oocytes/ovary were documented.