Publications by authors named "Abdalla A Elshereef"
Cyclic dimeric GMP (c-di-GMP) is a widespread bacterial second messenger that controls a variety of cellular functions, including protein and polysaccharide secretion, motility, cell division, cell development, and biofilm formation, and contributes to the virulence of some important bacterial pathogens. While the genes for diguanylate cyclases and c-di-GMP hydrolases (active or mutated) can be easily identified in microbial genomes, the list of c-di-GMP receptor domains is quite limited, and only two of them, PliZ and MshEN, are found across multiple bacterial phyla. Recently, a new c-di-GMP receptor protein, named CdgR or ComFB, has been identified in cyanobacteria and shown to regulate their cell size and, more recently, natural competence.
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- SbtB is a protein in cyanobacteria that plays a key role in regulating the carbon-concentrating mechanism (CCM) and interacts with adenyl nucleotides, featuring a redox-sensitive loop (R-loop) that changes based on the cell's light exposure.
- Research on mutants of SbtB in Synechocystis sp. PCC 6803 revealed that the R-loop helps the organism adapt to varying light conditions by affecting how SbtB binds to adenyl nucleotides and associates with membranes, which is crucial for optimal CCM function.
- The findings suggest that the redox state of the R-loop adds an important layer of regulation to SbtB, integrating carbon signaling with light
High cell densities for transient transfection with polyethyleneimine (PEI) can be used for rapid and maximal production of recombinant proteins. High cell densities can be obtained by different cultivation systems, such as batch or perfusion systems. Herein, densities up to 18 million cells/mL were obtained by centrifugation for transfection evaluation.
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