Deciphering the Kinetic Study of Sodium Dodecyl Sulfate on Ag Nanoparticle Synthesis Using Flower Extract as a Reducing Agent.

Silver nanoparticles (Ag NPs) were synthesized using flower petal extract (CSFE) as a reducing agent for the first time. In its presence and absence, the correlative effects of the anionic surface-active agent sodium dodecyl sulfate (SDS) were studied with respect to the development and texture of Ag NPs. Under different reagent compositions, the Ag NPs were inferred by localized surface plasmon resonance peaks between 419 and 455 nm.

Refolding of urea denatured cytochrome c: Role of hydrophobic tail of the cationic gemini surfactants.

The refolding of urea denatured horse heart cytochrome c (h-cyt-c) under the influence of ester based cationic gemini surfactants [ethane-1, 2-diyl bis(N, N-dimethyl-N-alkylammoniumacetoxy) dichlorides] 16-E2-16, 14-E2-14 and 12-E2-12 (n-E2-n) was performed by using UV-visible, fluorescence and circular dichroism (CD) spectroscopic techniques. We found that n-E2-n geminis promote the formation of molten globule (MG) like state upon addition into the urea denatured h-cyt-c. The comparative study of refolding of denatured h-cyt-c with n-E2-n, cationic gemini surfactant show stabilization of MG-like state influenced by hydrophobic interactions.

Hydrogen bonding-assisted interaction between amitriptyline hydrochloride and hemoglobin: spectroscopic and molecular dynamics studies.

Herein, we have explored the interaction between amitriptyline hydrochloride (AMT) and hemoglobin (Hb), using steady-state and time-resolved fluorescence spectroscopy, UV-visible spectroscopy, and circular dichroism spectroscopy, in combination with molecular docking and molecular dynamic (MD) simulation methods. The steady-state fluorescence reveals the static quenching mechanism in the interaction system, which was further confirmed by UV-visible and time-resolved fluorescence spectroscopy. The binding constant, number of binding sites, and thermodynamic parameters viz.

Urea-induced binding between diclofenac sodium and bovine serum albumin: a spectroscopic insight.

We investigated the interaction of diclofenac sodium (Dic.Na) with bovine serum albumin (BSA) in the absence and presence of urea using different spectroscopic techniques. A fluorescence quenching study revealed that the Stern-Volmer quenching constant decreases in the presence of urea, decreasing further at higher urea concentrations.

Spectroscopic and molecular modelling analysis of the interaction between ethane-1,2-diyl bis(N,N-dimethyl-N-hexadecylammoniumacetoxy)dichloride and bovine serum albumin.

Several spectroscopic approaches namely fluorescence, time-resolved fluorescence, UV-visible, and Fourier transform infra-red (FT-IR) spectroscopy were employed to examine the interaction between ethane-1,2-diyl bis(N,N-dimethyl-N-hexadecylammoniumacetoxy)dichloride (16-E2-16) and bovine serum albumin (BSA). Fluorescence studies revealed that 16-E2-16 quenched the BSA fluorescence through a static quenching mechanism, which was further confirmed by UV-visible and time-resolved fluorescence spectroscopy. In addition, the binding constant and the number of binding sites were also calculated.

Recent advances in the applications of ionic liquids in protein stability and activity: a review.

Room temperatures ionic liquids are considered as miraculous solvents for biological system. Due to their inimitable properties and large variety of applications, they have been widely used in enzyme catalysis and protein stability and separation. The related information present in the current review is helpful to the researchers working in the field of biotechnology and biochemistry to design or choose an ionic liquid that can serve as a noble and selective solvent for any particular enzymatic reaction, protein preservation and other protein based applications.

Spectroscopic and docking studies on the interaction between pyrrolidinium based ionic liquid and bovine serum albumin.

The interaction of synthesized ionic liquid, 1-butyl-1-methyl-2-oxopyrrolidinium bromide (BMOP) and bovine serum albumin (BSA) was investigated using UV-Vis, FT-IR, steady state and time resolved fluorescence measurements and docking studies. Steady state spectra revealed that BMOP strongly quenched the intrinsic fluorescence of BSA through dynamic quenching mechanism. The corresponding thermodynamic parameters; Gibbs free energy change (ΔG), entropy change (ΔS) and enthalpy change (ΔH) showed that the binding process was spontaneous and entropy driven.

Role of 1-methyl-3-octylimidazolium chloride in the micellization behavior of amphiphilic drug amitriptyline hydrochloride.

The mixed micellization behaviour of amitriptyline hydrochloride (AMT) with ionic liquid (IL) 1-methyl-3-octylimidazolium hydrochloride, [C8mim][Cl], have been investigated using electrical conductivity, at different temperatures. The non-ideal behaviour (i.e.

Interaction of amphiphilic drugs with human and bovine serum albumins.

To know the interaction of amphiphilic drugs nortriptyline hydrochloride (NOT) and promazine hydrochloride (PMZ) with serum albumins (i.e., human serum albumin (HSA) and bovine serum albumin (BSA)), techniques of UV-visible, fluorescence, and circular dichroism (CD) spectroscopies are used.

Effect of ionic liquid on activity, stability, and structure of enzymes: a review.

Ionic liquids have shown their potential as a solvent media for many enzymatic reactions as well as protein preservation, because of their unusual characteristics. It is also observed that change in cation or anion alters the physiochemical properties of the ionic liquids, which in turn influence the enzymatic reactions by altering the structure, activity, enatioselectivity, and stability of the enzymes. Thus, it is utmost need of the researchers to have full understanding of these influences created by ionic liquids before choosing or developing an ionic liquid to serve as solvent media for enzymatic reaction or protein preservation.

Spectroscopic approach of the interaction study of amphiphilic drugs with the serum albumins.

The interaction of the amphiphilic drugs, i.e., amitriptyline hydrochloride (AMT) and promethazine hydrochloride (PMT), with serum albumins (i.

Mixed micellization of antidepressant drug amitriptyline hydrochloride with cationic surfactants.

The surface tension measurements have been carried out on aqueous solutions of antidepressant drug amitriptyline hydrochloride with six cationic surfactants (decyl-, dodecyl-, tetradecyl-, cetyltrimethylammonium bromide, cetylpyridinium bromide and cetylpyridinium chloride) at different mole fractions to study the surface and micellar properties at the interface as well as in the micelles. The properties studied include critical micelle concentration (cmc), Gibbs surface excess (Gamma(max)), minimum head group area at the air/water interface (A(min)), free energy of micellization (DeltaG(m)( composite function)), and standard Gibbs energy of adsorption (DeltaG(ads)( composite function)), while synergistic behavior of drug-surfactant binary mixtures was analysed using Clint's and Rubingh's models. Deviations of cmc from cmc(*) and X(1)(m) from X(1)(ideal) indicate the synergistic behavior (i.