Informational molecules in amoebae studies of template utilization by the DNA polymerase activities of Amoeba discoides.

Studies have shown that certain strain-specific characters of large, free-living amoebae may be influenced by the microinjection of non-homologous low molecular weight RNAs. To investigate the mechanisms involved in 'information' transfer, the template preferences of partially purified DNA polymerase activities isolated from Amoeba discoides have been studied. After passage through Sephadex G-200, DNA polymerase activities from whole homogenates could utilize both 'activated' calf thymus DNA and the synthetic ribohomopolymer poly rA oligo d(pT)10 as templates.

Absence of low molecular weight DNA polymerase activity from the nuclei of Amoeba discoides.

Amoeba discoides nuclear protein partially purified by passage through Sephadex G-200 showed 3 high-mol.-wt. DNA polymerase activities which eluted in and just following the void volume.

Amoxycillin and co-trimoxazole in presumed viral respiratory infections of childhood: placebo-controlled trial.

A double-blind randomized controlled trial of amoxycillin, co-trimoxazole, and placebo was conducted on 197 children presenting with presumed viral respiratory infections. Routine throat swabs were taken to exclude streptococcal diseases. The three disease categories studied--nasopharyngitis, pharyngotonsillitis, and bronchitis (including laryngotracheobronchitis)--showed a generally similar pattern of resolution irrespective of treatment.

Duodenal intussusception secondary to Brunner's gland hamartoma. A case report.

Intussusception of the duodenum is a relatively rare event, and usually results from the presence of benign tumors. A case of duodenal intussusception secondary to Brunner's gland hamartoma is described and its differential diagnosis discussed.

Treatment of urinary-tract infection with a single dose of amoxycillin.

31 patients with a urinary infection were treated with a single oral dose (3 g for adults and 100 mg/kg for children) of amoxycillin; 23 (74%) were cured. 20 women and 26 children were then randomly allocated to either a single dose of amoxycillin of a 5- to 7-day conventional course of the same antibiotic. The results of both treatment regimens were comparable.

The neonatal colon: an anatomic approach to plain films.

An appreciation of haustral anatomy as well as the anatomic relationships of the colon serve as the basis of plain film colon identification in the patient beyond infancy. An evaluation of the newborn colon in the normal state and with low colonic obstruction indicates that these principles apply here as well. Newborns with suspected intestinal obstruction should be studied in multiple projections early in their clinical course.

Possible causes of complications of myelography with water-soluble contrast medium.

The mechanisms of CSF formation and absorption have been reviewed. The conditions that alter the rate of CSF formation and absorption may change the concentration of contrast in the cranial cisterns and the rate of passage through the cisterns. The significance of these alterations in relation to the complications of myelography with water-soluble contrast media is discussed.

Necroqizing enterocolitis with pneumatosis intestinalis in systemic lupus erythematosus and polyarteritis.

Pneumatosis intestinalis was encountered in association with fatal necrotizing enterocolitis in systemic lupus erythematosus (SLE) and polyarteritis nodosa. The radiologic identification of mottled, bubbly, and linear collections of intramural intestinal gas distinguish this ominous complication from benign pneumatosis cystoides intestinalis. In the setting of intestinal vasculitis due to SLE or polyarteritis nodosa, these characteristic radiologic features indicate necrotizing enterocolitis.

Fungal aneurysms of intracranial vessels.

Intracranial fungal aneurysms arise from major cerebral arteries. Fungi directly invade vessel walls from the luminal surface (fungal emboli) or from the adventitia (fungal meningitis). The vasa vasorum are free of fungi.

Neonatal bacteriuria: a prospective study in 1,460 infants.

In a prospective study of 1,460 virtually consecutive neonates 14 (1%) were found to have bacteriuria, established by bladder puncture. Eleven were boys and three girls. Five of the infants with bacteriuria had symptoms suggestive of infection and were treated with antibiotics.

Bacteriuria in pregnancy and infection in amniotic fluid and infant.

Women with asymptomatic bacteriuria during pregnancy had sterile amniotic fluid at the time of delivery. There was no evidence that maternal urinary infection was associated with infection in the infant.

Transient asymptomatic bacteriuria in infancy.

Spontaneous clearing of bacteriuria occurred in three infants during the neonatal and early infancy period. In all cases, the presence of bacteria was confirmed by bladder puncture, no signs of clinical infection were present, and no treatment was given. The same host defence mechanisms as exist in adults were probably responsible for this phenomenon.