Transposition is modulated by a diverse set of host factors in Escherichia coli and is stimulated by nutritional stress.

The role of host factors in regulating bacterial transposition has never been comprehensively addressed, despite the potential consequences of transposition. Here, we describe a screen for host factors that influence transposition of IS903, and the effect of these mutations on two additional transposons, Tn10 and Tn552. Over 20,000 independent insertion mutants were screened in two strains of Escherichia coli; from these we isolated over 100 mutants that altered IS903 transposition.

Genetic evidence that GTP is required for transposition of IS903 and Tn552 in Escherichia coli.

Surprisingly little is known about the role of host factors in regulating transposition, despite the potentially deleterious rearrangements caused by the movement of transposons. An extensive mutant screen was therefore conducted to identify Escherichia coli host factors that regulate transposition. An E.

Structure of the CoA transferase from pig heart to 1.7 A resolution.

Succinyl-CoA:3-ketoacid CoA transferase (SCOT; EC 2.8.3.