Decoding transaminase motifs: Tracing the unknown patterns for enhancing the accuracy of computational screening methodologies.

Transaminases, enzymes known for their amino group transfer capabilities, encompass four distinct subfamilies: D-alanine transaminase (DATA), L-selective Branched chain aminotransferase (BCAT), and 4-amino-4-deoxychorismate lyase (ADCL) and R-selective aminotransferase (RATA). RATA enzymes are particularly valuable in biocatalysis for synthesizing chiral amines and resolving racemic mixtures, yet their identification in sequence databases is challenging due to the lack of robust motif-based screening methods. Constructing a sequence dataset of transaminases, and categorizing them to various subfamilies, the conserved motifs are screened over the experimentally known ones, and the novel motifs are explored.