Study on socio-economic status and farming practices of fish farmers in Pachrauta Municipality, Bara, Nepal.

An interview-based questionnaire survey was done in 2021 to assess the socio-economic conditions, farming practices adopted, and problems faced by fish farmers and to analyze the cost and benefits of fish farming in the Fish super zone area of Pachrauta Municipality, Bara district. A simple random sampling technique was used to select 60 respondents for the study and the information collected was analyzed and processed by MS-Excel and SPSS. The majority of respondents were male, belonged to the age group of 25-36 years, and had a secondary level of school education.

Effect of different doses of triacontanol on growth and yield of kohlrabi ( L. var. ).

Triacontanol (TRIA), an endogenous plant growth regulator, promotes various metabolic activities in plants, resulting in improved growth and development in kohlrabi. The objective of this study was to assess the effect of different doses of triacontanol on the growth and yield of kohlrabi. This study was carried out in a randomized complete block design (RCBD) with five replications at Purkot Daha, Gulmi, Nepal, from October 2020 to January 2021.

Peptidomic analysis of healthy and subclinically mastitic bovine milk.

A variety of proteases release hundreds of endogenous peptide fragments from intact bovine milk proteins. Mass spectrometry-based peptidomics allows for high throughput sequence assignment of a large number of these peptides. Mastitis is known to result in increased protease activity in the mammary gland.

A peptidomic analysis of human milk digestion in the infant stomach reveals protein-specific degradation patterns.

In vitro digestion of isolated milk proteins results in milk peptides with a variety of actions. However, it remains unclear to what degree protein degradation occurs in vivo in the infant stomach and whether peptides previously annotated for bioactivity are released. This study combined nanospray LC separation with time-of-flight mass spectrometry, comprehensive structural libraries, and informatics to analyze milk from 3 human mothers and the gastric aspirates from their 4- to 12-d-old postpartum infants.

Peptidomic profile of milk of Holstein cows at peak lactation.

Bovine milk is known to contain naturally occurring peptides, but relatively few of their sequences have been determined. Human milk contains hundreds of endogenous peptides, and the ensemble has been documented for antimicrobial actions. Naturally occurring peptides from bovine milk were sequenced and compared with human milk peptides.