Publications by authors named "Aaron Volk"
Coronaviruses (CoVs) encode multiple interferon (IFN) antagonists that modulate the host response to virus replication. Here, we evaluated the host transcriptional response to infection with murine coronaviruses encoding independent mutations in one of two different viral antagonists, the deubiquitinase (DUB) within nonstructural protein 3 or the endoribonuclease (EndoU) within nonstructural protein 15. We used transcriptomics approaches to compare the scope and kinetics of the host response to the wild-type (WT), DUBmut, and EndoUmut viruses in infected macrophages.
View Article and Find Full Text PDFArticle Synopsis
- Investigating type I feline coronaviruses (FCoVs) in tissue culture is essential for understanding their virology and how they interact with hosts, but these strains have difficulty adapting to cell cultures.
- The study characterizes the replication and plaque formation of type I strain FIPV Black in a specialized cell line (Fcwf-4 CU), finding that high virus levels can be obtained by 20 hours post-infection.
- It concludes that Fcwf-4 CU cells can be effectively used for standardized plaque assays and are morphologically and functionally different from other cell types, showing reduced sensitivity to interferon.