Low-level laser therapy activates NF-kB via generation of reactive oxygen species in mouse embryonic fibroblasts.

Background: Despite over forty years of investigation on low-level light therapy (LLLT), the fundamental mechanisms underlying photobiomodulation at a cellular level remain unclear.

Methodology/principal Findings: In this study, we isolated murine embryonic fibroblasts (MEF) from transgenic NF-kB luciferase reporter mice and studied their response to 810 nm laser radiation. Significant activation of NF-kB was observed at fluences higher than 0.

Effects of 810-nm laser on murine bone-marrow-derived dendritic cells.

Objective: The purpose of this study was to investigate the effect of 810-nm low level laser therapy (LLLT) on dendritic cells (DC) in vitro.

Background Data: LLLT can enhance wound healing and increase cell proliferation and survival, and is used to treat inflammatory conditions. However there are reports that LLLT can stimulate leukocytes and could therefore be pro-inflammatory.

Photodynamic therapy as consolidation treatment for primary cutaneous adenoid cystic carcinoma: a case report.

Objective: The purpose of this case report was to evaluate the efficacy of photodynamic therapy (PDT) for a patient with primary cutaneous adenoid cystic carcinoma (PCACC).

Materials And Methods: A 53-year-old female patient presented with a lesion at the pre-auricular area. After performing a local resection along a margin of 0.

The influence of temperature on 5-aminolevulinic acid-based photodynamic reaction in keratinocytes in vitro.

Background/purpose: Based on the observation that increasing skin temperature could improve 5-aminolevulinic acid (ALA) penetration and accumulation of protoporphyrin IX (PpIX) in the ALA-based photodynamic therapy (PDT), this study was designed to investigate how temperature change varied the therapeutic effect of ALA-based PDT in vitro.

Methods: HaCat cells were cultured with or without ALA at various temperatures. ALA uptake and PpIX accumulation were analyzed before laser irradiation as the baseline.

Biphasic dose response in low level light therapy.

The use of low levels of visible or near infrared light for reducing pain, inflammation and edema, promoting healing of wounds, deeper tissues and nerves, and preventing cell death and tissue damage has been known for over forty years since the invention of lasers. Despite many reports of positive findings from experiments conducted in vitro, in animal models and in randomized controlled clinical trials, LLLT remains controversial in mainstream medicine. The biochemical mechanisms underlying the positive effects are incompletely understood, and the complexity of rationally choosing amongst a large number of illumination parameters such as wavelength, fluence, power density, pulse structure and treatment timing has led to the publication of a number of negative studies as well as many positive ones.