Publications by authors named "A-li Chai"

Article Synopsis
  • Purple stem mustards, cultivated in the Yangtze River Valley, are facing a serious threat from a soft rot disease, resulting in 20-30% plant loss and severe economic impact in Wuhan, China as of February 2023.
  • Infected plants show symptoms like water-soaked bases, foul odors, and pus-like discharges, eventually leading to death.
  • Researchers isolated 15 strains of bacteria from affected plants, identifying three representative strains through biochemical tests and genetic analysis, aiming to determine the specific cause of the soft rot outbreak.
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  • Lettuce, a cool-season crop from the Asteraceae family, was affected by a leaf disease in Beijing, China, showing dark lesions and a disease incidence of 10-40%.
  • Infected leaves exhibited translucent spots that developed into large necrotic areas, prompting a series of tests on bacteria isolated from the samples.
  • Pathogenicity tests on lettuce confirmed the disease symptoms within 7-10 days after exposure to specific bacterial isolates, which were then analyzed genetically to determine their identity.
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Coating seeds with biocontrol agents represents an effective approach for managing soil-borne plant diseases. However, improving the viability of biocontrol microorganisms on the seed surface or in the rhizosphere remains a big challenge due to biotic and abiotic stresses. In this work, we developed a microbial seed coating strategy that uses sporopollenin exine capsules (SECs) as carriers for the encapsulation of the biofilm-like biocontrol bacteria.

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  • Broccoli, a valuable vegetable in China, is facing a serious leaf spot disease that has caused about 60% incidence in a Weifang City field, leading to significant economic impacts.
  • *The disease manifests as dark, circular spots on leaves, which can develop into holes due to tissue decay.
  • *Lab analysis of affected samples revealed 12 similar bacterial strains, which were characterized as Gram-negative, aerobic, and rod-shaped, with the ability to utilize various sugars.
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Microbial seed coatings serve as effective, labor-saving, and ecofriendly means of controlling soil-borne plant diseases. However, the survival of microbial agents on seed surfaces and in the rhizosphere remains a crucial challenge. In this work, we embedded a biocontrol bacteria (Bacillus subtilis ZF71) in sodium alginate (SA)/pectin (PC) hydrogel as a seed coating agent to control Fusarium root rot in cucumber.

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Article Synopsis
  • In April 2023, severe soft rot symptoms were noted in commercial broccoli fields in Yunnan, China, leading to over 50% yield loss with plants showing rotten roots and emitting foul odors.
  • To identify the cause, symptomatic stems were sterilized, plated on nutrient agar, and three bacterial isolates were analyzed, showing characteristics aligned with Pectobacterium polaris.
  • Genomic DNA was extracted and analyzed through sequencing of the 16S rRNA and nine housekeeping genes, confirming the isolates belonged to the genus Pectobacterium, as verified by BLASTn analysis.
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  • - The Mongolian snake gourd, a valuable herbal medicine in China, is facing a serious root rot issue on a 20-ha farm in Zhenjiang City, leading to a 70% disease incidence and a 50% reduction in production as of 2021-2022.
  • - Symptoms of the disease include yellow mottling on new leaves, wilting of upper leaves, and browning at the base of the stem, eventually causing the plants to die.
  • - Researchers isolated a specific fungus from infected plants, leading to the identification of a representative strain for further analysis, revealing distinct conidial features that could help in understanding the disease.
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Water convolvulus (Ipomoea aquatica Forsk.), a member of the Convolvulaceae family, is an important tropical vegetable cultivated in China (Liu et al. 2017).

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In February 2020, the common symptoms of water-soaked spots on Cucurbita pepo L. cotyledon were observed in Guangrao county in Shandong province, China. Field investigation showed that 40% of the Cucurbita pepo cotyledons in an area of approximately 0.

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  • Celery crops, commonly grown in the Mediterranean, Europe, and parts of Asia, were affected by a disease showing leaf spots and stem lesions during the spring of 2014 in Beijing and Sichuan Province, China.
  • Symptoms included subcircular leaf spots with brown centers and necrotic areas on the stems, and surveys indicated a disease incidence of 30%-60% in the surveyed greenhouses.
  • The pathogen was identified as similar to Cercospora apiicola based on morphological characteristics and DNA analysis, involving amplification of specific gene regions for further study.
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Okra ( (L.) Moench) has gained more popularity as an economically significant plant for its nutritional and medicinal value, especially in China. During 2014-2016, the root disease of okra was discovered in four okra commercial fields surveyed in China.

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  • - Severe root rot of cabbages was identified in China in 2015, characterized by root rot and wilting leaves.
  • - A specific fungus was consistently isolated from diseased cabbages, and its identity was confirmed through both morphological features and molecular analysis.
  • - This study marks the first documented instance of this fungus causing root rot in cabbages, both in China and globally.
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Identification of plant-pathogenic fungi is time-consuming due to cultivation and microscopic examination and can be influenced by the interpretation of the micro-morphological characters observed. The present investigation aimed to create a simple but sophisticated method for the identification of plant-pathogenic fungi by Fourier transform infrared (FTIR) spectroscopy. In this study, FTIR-attenuated total reflectance (ATR) spectroscopy was used in combination with chemometric analysis for identification of important pathogenic fungi of horticultural plants.

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Clubroot, caused by Plasmodiophora brassicae, is considered the most devastating soilborne disease in Brassica crops. It has emerged as a serious disease threatening the cruciferous crop production industry in China. Nowadays, the detection techniques for P.

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Fourier transform infrared (FTIR) attenuated total reflectance (ATR) spectroscopy was used in combination with multivariate statistic analysis for identification of soil-borne fungi that causes severe economic damage to agriculture: Fusarium monili forme, Fusarium semitectum, Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Sclerotinia sclerotiorum, Pythium aphanidermatum and Phytophthora capsici. The original FTIR spectra were normalized, and the second derivatives were calculated, from which the peak wave numbers showing greatest variability were selected: 2924, 2854, 1745, 1641, 1547, 1466, 1406, 1376, 1306, 1240, 1201, 1152, 1109 and 1028 cm(-1). To discriminate different fungal strains, canonical discriminant analysis and cluster analysis were performed at these characteristic wave numbers.

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Fourier transform infrared spectroscopy (FTIR) technique was applied in the early detection of corynespora spot on cucumber leaves while the symptom had not appeared. The cucumber leaves were inoculated with Corynespora cassiicola. By observing the changes in the FTIR spectra of infected cucumber leaves at various times of post-infection, three sensitive bands, 1 735, 1 545 and 1 240 cm(-1) were selected for the identification of cucumber corynespora leaf spot.

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Fourier transform attenuated total reflection infrared spectroscopy (FTIR-ATR) has been a novel technical procedure to identify and classify microorganisms in recent years. In the present study, Fourier transform infrared spectroscopy(FTIR) in combination with an attenuated total reflection (ATR) unit were used to discriminate important plant-destroying fungi. Mycelia of 17 fungal strains belonging to 14 different species were grown on potato dextrose agar (PDA) plants and subjected to FTIR-ATR measurements.

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Hyperspectral imaging (400-720 nm) and discriminate analysis were investigated for the detection of normal and diseased cucumber leaf samples with powdery mildew (Sphaerotheca fuliginea), angular leaf spot (Pseudomopnas syringae), downy mildew (Pseudoperonospora cubensis), and brown spot (Corynespora cassiicola). A hyperspectral imaging system was es tablished to acquire and pre-process leaf images, as well as to extract leaf spectral properties. Owing to the complexity of the original spectral data, stepwise discriminate and canonical discriminate were executed to reduce the numerous spectral information, in order to decrease the amount of calculation and improve the accuracy.

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