Publications by authors named "A van Rie"
Bedaquiline (BDQ) is crucial for the treatment of rifampicin-resistant tuberculosis, yet resistance threatens its effectiveness, mainly linked to mutations in the () gene. While frameshift mutations are thought to produce non-functional proteins, we hypothesize that they can result in conserved proteins through late-stop codons or alternative reading frames and remain BDQ susceptible. We extracted 512 isolates harboring frameshift mutations in from the World Health Organization (WHO) catalog and 68 isolates with minimum inhibitory concentration (MIC) in mycobacterial growth indicator tube (MGIT) through a literature review.
View Article and Find Full Text PDFBackground: Accurate diagnosis of bedaquiline (BDQ) resistance remains challenging. A Bayesian approach expresses this uncertainty as a probability of BDQ resistance (prBDQ) with a 95% credible interval. We investigated how prBDQ information influences BDQ prescribing decisions.
View Article and Find Full Text PDFArticle Synopsis
- Rifampicin resistant tuberculosis is a major global health issue, with significant limitations in current treatment practices due to inadequate drug susceptibility testing (DST) information.
- The study developed a clinical decision support system (CDSS) that utilizes machine learning to recommend optimal individualized treatment regimens based on extensive data from expert feedback.
- Although the CDSS was found to be user-friendly and beneficial in clinical settings, its effectiveness is hindered by challenges such as adherence to national treatment guidelines and the need for better digital infrastructure and training for healthcare providers.
Article Synopsis
- Oxford Nanopore Technologies (ONT) has introduced R10 flowcells and V14 rapid sequencing chemistry, which were evaluated for sequencing Mycobacterium tuberculosis (Mtb) DNA from clinical samples, yielding mixed results in terms of quality and quantity.
- In initial runs, sequencing generated a moderate amount of data (1.94 Gb, 214k reads), but only 49% of reads met acceptable quality standards (Phred score >8). Subsequent experiments with a clean-up step improved yield to 2.38 Gb and 66% quality, yet still fell short of optimal performance.
- The study indicates that further optimization of ONT protocols is necessary, as the data did not meet the