Publications by authors named "A van Oudenaarden"
Translation is one of the most energy-intensive processes in a cell and, accordingly, is tightly regulated. Genome-wide methods to measure translation and the translatome and to study the complex regulation of protein synthesis have enabled unprecedented characterization of this crucial step of gene expression. However, technological limitations have hampered our understanding of translation control in multicellular tissues, rare cell types and dynamic cellular processes.
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- The mammalian pancreas has three key parts: exocrine acini and ducts, along with endocrine islets, all originating from a common progenitor during development.
- Researchers created 18 human fetal pancreas organoid lines from samples between 8-17 weeks of gestation, with four lines showing the ability to produce all three cell types while thriving in culture for over two years.
- Single-cell RNA sequencing revealed LGR5 cells as crucial developmental stem cells, indicating that these organoids are capable of long-term growth and can differentiate into acinar, ductal, and endocrine cells.
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- Long-term issues in chromatin assembly during DNA replication can significantly impact how cells maintain their epigenetic information and decide their fate.
- Researchers studied the effects of quickly removing a key protein called CAF-1, which is crucial for building chromatin, by using advanced techniques like single-cell genomics and live microscopy.
- The loss of CAF-1 slows DNA replication, makes new DNA more accessible, triggers a unique cellular response that reduces histone mRNA levels, and ultimately leads to cell-cycle arrest influenced by p53, highlighting the immediate consequences of faulty chromatin assembly.
Human development relies on the correct replication, maintenance and segregation of our genetic blueprints. How these processes are monitored across embryonic lineages, and why genomic mosaicism varies during development remain unknown. Using pluripotent stem cells, we identify that several patterning signals-including WNT, BMP, and FGF-converge into the modulation of DNA replication stress and damage during S-phase, which in turn controls chromosome segregation fidelity in mitosis.
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