Publications by authors named "A de Fraissinette"
At its 25th meeting the ECVAM Scientific Advisory Committee (ESAC) unanimously endorsed that the SkinEthic Reconstructed Human Epidermis (RHE) model could be used for distinguishing between corrosive and non-corrosive chemicals within the context of the Organisation Economic for Co-operation and Development (OECD) test guideline, TG 431 (ESAC 16-17 November 2006). Both test method development and multi-center study were performed using 0.63 cm(2) RHE tissue samples.
View Article and Find Full Text PDFIn vitro assessment of eye irritancy using the Reconstructed Human Corneal Epithelial SkinEthic HCE model: application to 435 substances from consumer products industry.
Toxicol In Vitro
March 2010
Article Synopsis
- The 7th amendment of the EU Cosmetics Directive banned animal testing for eye irritation in cosmetics starting March 11th, 2009, prompting the search for alternative testing methods.
- Researchers utilized the SkinEthic HCE model to assess the eye irritancy of 102 cosmetic substances, establishing a prediction model that effectively classified irritants and non-irritants with high sensitivity and specificity.
- The method was further validated with an expanded set of 435 substances, achieving over 82% accuracy and demonstrating its potential as a reliable in vitro tool for predicting eye irritancy, leading to collaborations for prospective validation.
The development of in vitro protocols able to discriminate skin irritants from non-irritants integrates the toxicologists' needs for reliable and robust in vitro tools for screening test substances. Based on EpiSkin test method, validated by ESAC (ECVAM Scientific Advisory Committee) in April 2007 as the Draize skin irritation replacement reference test method, we present and discuss here the results obtained by adapting protocols to the SkinEthic Reconstructed Human Epidermis (RHE) model. The main adaptations of the validated reference protocol consists in a modulated exposure time (15, 42 or 60min) followed by a rinsing step and a 42h post-incubation period before quantitative measurement of cell viability by MTT reduction.
View Article and Find Full Text PDFNickel is a ubiquitous and virtually unavoidable environmental pollutant and occupational hazard, but its molecular and cellular effects are not well understood. Human epidermal keratinocytes are the sentinel and the primary target for nickel. We treated with nickel salts skin equivalents containing differentiating epidermal keratinocytes grown on air-liquid interface in standard cell culture conditions.
View Article and Find Full Text PDFThe skin tolerability of the tubulin polymerisation inhibitor LAV694 was compared to that of 5% 5-fluorouracil (5-FU) and 0.5% podophyllotoxin in vitro using a human reconstructed epidermis (HRE), and in vivo using minipigs. Topical treatment of HRE for 1 or 3 days with a 0.
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