Genetic sequencing for surveillance of drug resistance in tuberculosis in highly endemic countries: a multi-country population-based surveillance study.

Background: In many countries, regular monitoring of the emergence of resistance to anti-tuberculosis drugs is hampered by the limitations of phenotypic testing for drug susceptibility. We therefore evaluated the use of genetic sequencing for surveillance of drug resistance in tuberculosis.

Methods: Population-level surveys were done in hospitals and clinics in seven countries (Azerbaijan, Bangladesh, Belarus, Pakistan, Philippines, South Africa, and Ukraine) to evaluate the use of genetic sequencing to estimate the resistance of Mycobacterium tuberculosis isolates to rifampicin, isoniazid, ofloxacin, moxifloxacin, pyrazinamide, kanamycin, amikacin, and capreomycin.

Alarming levels of multidrug-resistant tuberculosis in Ukraine: results from the first national survey.

Setting: The true prevalence of multidrug-resistant tuberculosis (MDR-TB) in Ukraine is not known. Available data are a decade old and limited to only one province.

Objective: To determine the prevalence of MDR-TB among new and previously treated TB cases in Ukraine and explore the risk factors associated with drug resistance.

[New selective agents for the isolation of Pseudomonas aeruginosa].

Two new selective media were prepared for the isolation of P. aeruginosa cultures from clinical material: RChZh medium with ramrod inhibitor and Zh medium including a selective complex (hydrazine sulfate, gramurin, ethonium, N-cetylpyridine chloride). The media are characterized by marked selective characteristics, simple to use, contain no unavailable reagents, inexpensive, and easy to prepare.

[The modelling of a Pseudomonas aeruginosa wound process].

P. aeruginosa wound infection was induced in white mice to test new preparations against P. aeruginosa.