Use of computer TV morphodensitometry to study epigenetic changes in blood lymphocytes from children affected by low-dose irradiation.

A novel method, computer TV morphodensitometry, was used to evaluate the effects of low-dose irradiation on peripheral blood lymphocytes from children affected by the Chernobyl disaster. This method uses digitized images to detect and measure changes in chromatin shape and density and to produce two-dimensional and three-dimensional pictures. Images can then be stored to create a video archive.

Interphase genome as the active space: chromatin dynamics during chick embryo chondrogenesis.

The rearrangement of the chromatin that takes place during cytodifferentiation was studied using TV image analysis in chick limb bud cartilage stained for DNA. The redistribution of the chromatin was compatible with the Rabl orientation: chromatin was extended radially from the centromeric ring to the telomere pole in young chondroblasts, and contracted back in ageing chondrocytes. The direction and gradient of this redistribution correlate with the changes in DNA content within the chromocentres formed by pericentromeric heterochromatin.

The chromatin network: image analysis of differentiating chick embryo chondrocytes.

The chromatin network was revealed in DNA stained cell imprints of developing chick embryo chrondrocytes using the recently developed method of TV image processing. The network consists of two classes of alveoles. The large alveoles, which accompany large chromocentres, are concentrated at the centromeric nuclear pole representing the most stable part of the network.

Accumulation of DNA within chromocentres of terminally differentiating chick embryo chondrocytes.

Automated television (TV) densitometric technique which allows the recognition and recording of chromatin compartments was applied to the study of chromatin rearrangement during chondrogenesis. Genetically active chondroblasts and inactive definite chondrocytes of E7 chick cartilage model, stained on the imprints for DNA, were a subject for the comparative study. Large chromatin granules with constant morphometric parameters, displaying positive staining for C-heterochromatin and identified as chromocentres, were found to accumulate 30% of cellular DNA, doubling its concentration during chondrogenic maturation.

The possibilities of the automated morphometry in the diagnosis of the hyperlipoproteidemia. Morphometry in the diagnosis of the hyperlipoproteidemias.

Following are the results of using the elaborated algorhythms of the structural analysis for the study of the form elements of the blood. A possibility was demonstrated to carry out a differential diagnosis of the familial hyperlipoproteidemia of the second type according to the quantitative structural parametres of the chromatin of the inter-phase nuclei of the lymphocytes of the peripheral blood. A method was developed of the automated morphometry of erythrocytes making it possible to assess the optical profile of erythrocytes according to 16 parametres.