[Possible mechanism of metabolic disturbance in gastrocnemius muscle after denervation].

Under complete denervation of m. gastrocnemius by crushing of sciatic nerve it was established that the intensity of oxygen free radicals formation increased 3 times, lipid peroxidation intensity 2 times, and the activity of antioxidative enzymes (glutathione reductase, superoxide dismutase) increased by 30-50%. Under these conditions the content of sarcolemmal neutral lipids increases and that of phospholipids decreases, proteins disintegrate, muscle atrophy increases.

[Ca2+ ion transport in the sarcoplasmic reticulum of the gastrocnemius muscles of white rats under treatment of the sciatic nerve with a colchicine solution].

In different time after application of 12.5 microM colchicine-inhibitor of exoplasmic current to the ischiadicus the quantity of amino acid residues of methionine and cysteine in the sarcoplasmic reticulum decreases, its protein content changes. The Ca(2+)-ATPase activity increases on the 3d day after colchicine application and decreases on the 14th day.

[Ca2+ metabolism in the sarcoplasmic reticulum of skeletal muscle after surgical denervation].

The ability of sarcoplasmic reticulum to regulate Ca(2+)-metabolism was studied at different terms after surgical denervation. The increase of Ca(2+)-ATP-ase activity, intensification of active Ca(2+)-accumulation under insignificant change of passive Ca(2+)-outflow from sarcoplasmic reticulum vesicles were observed 3-days after denervation. At more remote terms 14 days and 28 days after denervation all these parameters decreased except for Km: Ca(2+)-ATP activity and active Ca2+ accumulation decreased below normal level.

[Effect of skeletal muscle atrophy on sarcolemma lipids and proteins].

It is shown in the experiments on rats that the atrophy of skeletal muscles which has developed as a result of disturbances of neurotrophic control leads to the changes in protein spectrum of sarcolemma, growth of activity of membrane-associated enzymes (Na+, K(+)-ATPase, Ca(2+)-ATPase), intensive functioning of the prooxidative and antioxidative system of the muscle fibers.

[Effect of combined anesthetics on the activity of various myocardium enzymes].

It is demonstrated by experiments with rabbits that the Ca2+-ATP-ase activity is stabilized when using combined anesthetics (diacetylcholine + halothane + N2O) as distinct from application of halothane. A decrease in the cholinesterase activity is less pronounced than under the halothane action but more than with the diacetylcholine application. A decrease in the Na+, K+-ATP-ase activity is observed with all types of anesthesia.