Case of Video-Assisted Thoracoplasty Application in Pulmonary Tuberculosis Treatment.

A patient with extensive fibrocavitary pulmonary tuberculosis and extensively drug-resistant Mycobacterium tuberculosis underwent bilateral video-assisted thoracic surgery thoracoplasty. An examination 4 years post-surgery revealed that the patient was in a satisfactory condition and able-bodied. We performed surgery according to our proposed video-assisted thoracic surgery thoracoplasty technique.

Randomized DNA libraries construction tool: a new 3-bp 'frequent cutter' TthHB27I/sinefungin endonuclease with chemically-induced specificity.

Background: Acoustic or hydrodynamic shearing, sonication and enzymatic digestion are used to fragment DNA. However, these methods have several disadvantages, such as DNA damage, difficulties in fragmentation control, irreproducibility and under-representation of some DNA segments. The DNA fragmentation tool would be a gentle enzymatic method, offering cleavage frequency high enough to eliminate DNA fragments distribution bias and allow for easy control of partial digests.

Thermostable proteins bioprocesses: The activity of restriction endonuclease-methyltransferase from Thermus thermophilus (RM.TthHB27I) cloned in Escherichia coli is critically affected by the codon composition of the synthetic gene.

Obtaining thermostable enzymes (thermozymes) is an important aspect of biotechnology. As thermophiles have adapted their genomes to high temperatures, their cloned genes' expression in mesophiles is problematic. This is mainly due to their high GC content, which leads to the formation of unfavorable secondary mRNA structures and codon usage in Escherichia coli (E.

[Personal e-cards for military personnel and military-medical information system].

The article presents main directions of activities of the medical service, dealing with implementation of personal electronic cards for military personnel, organizing the process of automation of medical service management, military and medical organizations and health care departments. The given article, reveals the on-going activity, concerning creation of the military-medical information system, which will unite all medical units, organizations, and governments into one information space.

Two-stage gene assembly/cloning of a member of the TspDTI subfamily of bifunctional restriction endonucleases, TthHB27I.

The Thermus sp. family of bifunctional type IIS/IIG/IIC restriction endonucleases (REase)-methyltransferases (MTase) comprises thermo-stable TaqII, TspGWI, TspDTI, TsoI, Tth111II/TthHB27I enzymes as well as a number of putative enzymes/open reading frames (ORFs). All of the family members share properties including a large protein size (ca.