A non-covalently bound redox indicator for electrochemical CRISPR-Cas12a and DNase I biosensors.

A rapid and accurate biosensor for detecting disease biomarkers at point-of-care is essential for early disease diagnosis and preventing pandemics. CRISPR-Cas12a is a promising recognition element for DNA biosensors due to its programmability, specificity, and deoxyribonuclease activity initiated in the presence of a biomarker. The current electrochemical CRISPR-Cas12a-based biosensors utilize the single-stranded DNA (ssDNA) self-assembled on an electrode surface and covalently modified with the redox indicator, usually methylene blue (MB).

Structure and inference in hypergraphs with node attributes.

Many networked datasets with units interacting in groups of two or more, encoded with hypergraphs, are accompanied by extra information about nodes, such as the role of an individual in a workplace. Here we show how these node attributes can be used to improve our understanding of the structure resulting from higher-order interactions. We consider the problem of community detection in hypergraphs and develop a principled model that combines higher-order interactions and node attributes to better represent the observed interactions and to detect communities more accurately than using either of these types of information alone.

Prosthetic rehabilitation of patients with maxillary oncology defects using zygomatic implants.

Purpose: Prosthetics for patients after oncological resection of the upper jaw is a complex problem associated with the physiological and anatomical separation of the oral cavity and the nasal/paranasal region. This study reports the clinical results of the use of the zygomatic implants for prosthetic rehabilitation in patients with maxillectomy due to upper jaw tumors.

Materials And Methods: The study included 16 patients who underwent prosthetic rehabilitation using a zygomatic implant after maxillectomy period from 2021 to 2023.

Investigation of the Strain-Stress Field in Nanoscale Multilayer Systems by the Phase Plane Method.

This paper presents the results of the study of stress relaxation fields, deformation, and temperature of the system of nanostructured multilayer coatings. In the work, a nonlinear relationship between strain and stress was used to take into account nonlinear effects in the mechanism of nanostructure formation. The paper assumes that a friction surface is provided by the self-organization of shear components: both stress and strain on the one hand, and temperature on the other.

A voltammetric study of nitrogenase MoFe-protein using low-potential electron transfer mediators.

The molybdenum-iron protein (MoFeP), a component of the enzyme nitrogenase, catalyzes the reduction of an array of small molecules, including N to NH. In microorganisms, during the catalytic cycle, MoFeP receives electrons from the obligate biological redox partner iron protein (FeP) in a process coupled to the hydrolysis of two MgATP per one electron transferred. Despite the favorable redox properties of the cofactors, the requirement of the MgATP hydrolysis significantly decreases the energy efficiency of MoFeP.