[Application of alpha-tocopherol acetate prevents swelling of brain slices during the autoblood clot action].

The swelling of olfactory cortex slices of the hypertensive SHR rats under the long autoblood action have been studied. The influence of a preincubation of slices with vitamins E, C and D on a degree of swelling have been detected by their weighing before and after exposure to autoblood. The water-soluble form of vitamin E have exerted a substantial antiswelling action exceeding the same of vitamin D, whereas vitamin C had no any effect.

[Edema in nervous tissue studied on model of hemorrhagic stroke in vitro].

The development of edema in the survival olfactory cortex slices under the long-term action of autoblood has been studied by monitoring the bioelectric activity of nervous cells. The level of disorder in electrogenesis of cells was revealed by comparing the focal potentials with their control values; the degree of the nervous tissue swelling in various periods of autoblood action was determined by weighing. In the model of hemorrhagic stroke, the dependence of edema growth on the level of activity of ionotropic glutamate receptors has been determined using the pharmacological blockade technique.

[Protective and antiedema effect of heat shock HSP70 protein in hemorrhagic stroke model in vitro].

An in vitro model of hemorrhagic stroke in live olfactory cortex slices under long-term influence of autoblood has been used and the development of edema in the samples has been studied with simultaneous monitoring of bioelectric activity of the nervous cells. Protection of the nervous cells in the olfactory cortex slices of the spontaneously hypertensive (SHR) rats from consequences of the hemorrhagic stroke was achieved by incubating brain slices for 20 min in glass vials with 1 ml of incubation solution containing heat shock protein HSP70 at a concentration of 10 mg/ml. Then the incubation medium was replaced by 3 ml of autoblood, the action of which on the nervous cells modeled the hemorrhagic stroke.

[Effect of heparin on the neuron bioelectric activity in a hemorrhagic stroke model in vitro].

The effect of heparin on the activity of cerebral neurons under conditions of hemorrhagic stroke model was studied in vitro using brain slices of hypertensive rats. The auto-blood induced blocking of the parameters of focal potentials in neurons. The treatment with heparin (25 IU/ml) prior to the auto-blood action prevented inhibition of the activity of ionotropic glutamatergic and GABAergic receptors.

Neuroprotective properties of l-carnosine in the brain slices exposed to autoblood in the hemorrhagic stroke model in vitro.

Neuroprotective properties of L-carnosine have been studied in our in vitro model on olfactory cortex slices of hypertensive rats under a long autoblood (blood clot) influence. Application of L-carnosine (5 mg/ml) on slices before autoblood influence leads to restoration of the activity of glutamatergic and GABA-ergic receptors inhibited in the presence of autoblood and interferes with swelling of slices. L-carnosine protects a bioelectric activity of nervous cells in case of long influence of autoblood and also renders an anti edema effect.