[Characteristics of aging skin].

The change in the population's age structure in most industrial countries, as in Germany, requires geriatric medicine to play an increasingly important role. Dermatology also has to meet the new challenges by expert discussion and ethical considerations. The physiological aging process is influenced by intrinsic and extrinsic factors and causes a variety of morphological and functional alterations in the skin.

Use of hyaluronidase for pharmacokinetic increase in bioavailability of intracutaneously applied substances.

Endogenous hyaluronidases in the human body have physiological and pathophysiological significance due to their enzymatic activity during the cleavage of hyaluronan. The potential of therapeutic use of testicular and recombinant hyaluronidase has been increasingly acknowledged and developed in recent years. Most relevant in practice is the co-application of hyaluronidase and other active substances in order to enhance their bioavailability within the tissue.

The isotridecanyl side chain of plusbacin-A3 is essential for the transglycosylase inhibition of peptidoglycan biosynthesis.

Plusbacin-A3 (pb-A3) is a cyclic lipodepsipeptide that exhibits antibacterial activity against multidrug-resistant Gram-positive pathogens. Plusbacin-A3 is thought not to enter the cell cytoplasm, and its lipophilic isotridecanyl side chain is presumed to insert into the membrane bilayer, thereby facilitating either lipid II binding or some form of membrane disruption. Analogues of pb-A3, [(2)H]pb-A3 and deslipo-pb-A3, were synthesized to test membrane insertion as a key to the mode of action.

Clinical trial for safety evaluation of hyaluronidase as diffusion enhancing adjuvant for infiltration analgesia of skin with lidocaine.

Background: Infiltration anesthesia of the skin is an analgesic procedure often practiced before minor surgical interventions or punctures. The addition of hyaluronidase is a possible option to improve the effectiveness of the local anesthetic with respect to expansion of effect.

Objective: To validate the safety of intracutaneous application of hyaluronidase as a lidocaine adjuvant.