Quantitative determination of the dopamine agonist lisuride in plasma using high-performance liquid chromatography with fluorescence detection.

An HPLC method for the determination of lisuride hydrogen maleate in plasma is described. After addition of ergotamine tartrate as internal standard, plasma is extracted with diethyl ether. Following evaporation of the solvent and redissolving in methanol the extract is injected on a silica HPLC column and lisuride is monitored by fluorescence detection using an excitation wavelength of 322 nm and an emission wavelength of 405 nm.

Delta-5-androstenediol and its sulphate in serum and urine of normal adults and patients with endocrine diseases.

Objectives: We evaluated the role of delta-5-androstenediol (adiol) and its sulphates in health and endocrine diseases.

Design: Serum and urine samples from healthy adult men and pre and post-menopausal women were analysed by gas chromatography-mass spectrometry to establish reference values. In patients who were either evaluated or treated for endocrine diseases, sequential serum samples were collected and analysed.

The determination of delta-5-androstenediol and its sulphate in serum and urine by gas chromatography-mass spectrometry.

Objectives: We developed an assay for delta-5-androstenediol (adiol) and delta-5-androstenediol-3-sulphate (adiol-3S) in serum and adiol and total adiol sulphate (adiol-S) in urine.

Design: An analytical procedure using HPLC and gas chromatography-mass spectrometry was devised and tested for its reliability.

Measurements: After addition of deuterated androstenediol as internal standard, serum and urine samples were extracted.

Determination of megestrol acetate and cyproterone acetate in serum of patients with advanced breast cancer by high-performance liquid chromatography.

A reversed-phase high-performance liquid chromatographic method with ultraviolet detection of megestrol acetate and cyproterone acetate in human sera is described. The proposed assay is linear up to 1400 ng/ml (r = 0.999) and has a detection limit of 5 ng/ml.