Identification of serum tRNA-derived small RNAs biosignature for diagnosis of tuberculosis.

The tRNA-derived small RNAs (tsRNAs) are a new class of non coding RNAs, which are stable in body fluids and can be used as potential biomarkers for disease diagnosis. However, the exact value of tsRNAs in the diagnosis of tuberculosis (TB) is still unclear. The objective of the present study was to evaluate the performance of the serum tsRNAs biosignature to distinguish between active TB, healthy controls, latent TB infection, and other respiratory diseases.

Activation of locus coeruleus noradrenergic neurons rapidly drives homeostatic sleep pressure.

Homeostatic sleep regulation is essential for optimizing the amount and timing of sleep for its revitalizing function, but the mechanism underlying sleep homeostasis remains poorly understood. Here, we show that optogenetic activation of locus coeruleus (LC) noradrenergic neurons immediately increased sleep propensity following a transient wakefulness, contrasting with many other arousal-promoting neurons whose activation induces sustained wakefulness. Fiber photometry showed that repeated optogenetic or sensory stimulation caused a rapid reduction of calcium activity in LC neurons and steep declines in noradrenaline/norepinephrine (NE) release in both the LC and medial prefrontal cortex (mPFC).

Head and neck automatic multi-organ segmentation on Dual-Energy Computed Tomography.

Background And Purpose: Deep-learning-based automatic segmentation is widely used in radiation oncology to delineate organs-at-risk. Dual-energy CT (DECT) allows the reconstruction of enhanced contrast images that could help with manual and auto-delineation. This paper presents a performance evaluation of a commercial auto-segmentation software on image series generated by a DECT.

In Vitro Biological Evaluation and In Silico Studies of a New Anthraquinone From Zingiber Cassumunar Roxb. in Type 2 Diabetes Management.

A novel diphenyl-anthraquinone compound, cassuquinone A (CA), was isolated from the rhizomes of Zingiber cassumunar. Structural elucidation was accomplished using detailed nuclear magnetic resonance and high-resolution mass spectroscopy-electrospray ionization techniques, revealing a symmetrical anthraquinone core with methoxylated aromatic rings. CA exhibited potent α-glucosidase (AR) inhibitory activity with an IC₅₀ of 11.