Publications by authors named "A Verdel"
Article Synopsis
- Transcriptional silencing by RNA interference (RNAi) involves a complex process that requires the presence of transcription, specifically in Schizosaccharomyces pombe where the Cryptic Loci Regulator complex (CLRC) plays a crucial role.
- CLRC, functioning as a cullin-ring E3 ligase, interacts with the E2 enzyme Ubc4, which modifies the histone methyltransferase Clr4, facilitating the shift from co-transcriptional to transcriptional gene silencing through different forms of histone methylation.
- The study highlights the importance of phase separation and the role of non-coding RNA in regulating this process, impacting other transcriptional
The mA epitranscriptomic mark is the most abundant and widespread internal RNA chemical modification, which through the control of RNA acts as an important factor of eukaryote reproduction, growth, morphogenesis and stress response. The main mA readers constitute a super family of proteins with hundreds of members that share a so-called YTH RNA binding domain. The majority of YTH proteins carry no obvious additional domain except for an Intrinsically Disordered Region (IDR).
View Article and Find Full Text PDFHere, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated fraction. We then detail an RNA immunoprecipitation procedure adapted to identify chromatin-associated RNA targets.
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- Heat stress triggers significant changes in gene expression, primarily involving the protein YTHDC1, which interacts with N-methyladenosine (mA) RNA modifications.
- YTHDC1 relocates within the genome during heat stress, enhancing the expression of heat shock proteins (HSPs) and leading to altered transcript processing for many genes.
- The study suggests that YTHDC1's accumulation in specific nuclear stress bodies helps regulate and time the overall gene expression response to heat stress.
To eliminate specific or aberrant transcripts, eukaryotes use nuclear RNA-targeting complexes that deliver them to the exosome for degradation. S. pombe MTREC, and its human counterpart PAXT, are key players in this mechanism but inner workings of these complexes are not understood in sufficient detail.
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