Publications by authors named "A Velegraki"

Article Synopsis
  • An emerging multidrug-resistant fungal pathogen presents challenges in healthcare due to high misidentification and treatment resistance, highlighting a need for better identification methods.
  • The study utilized Raman spectroscopy on fungal cultures grown on modified Dixon's agar to differentiate among species, identifying key spectral markers that distinguish them into subgroups and using principal component analysis (PCA) for further differentiation.
  • The research also revealed a unique spectral signature for the modified agar, showcasing Raman spectroscopy's ability for real-time, non-destructive identification, despite the challenges posed by spectral similarity and variability among closely related fungi.
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and the recently separated acremonium-like genera, such as , are emerging causes of opportunistic disease in humans, mainly post-traumatic infections in immunocompetent hosts, but also invasive infections in immunocompromised patients, such as those undergoing transplantation. has emerged as the major pathogenic species in humans, implicated mainly in nail but also in disseminated and organ specific infections. In this first study of acremonium-like clinical isolates in Greece, 34 isolates were identified and typed by sequencing the internal transcribed spacer, and their antifungal susceptibility was determined by a modified CLSI standard M38 3rd Edition method for filamentous fungi.

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In the field of molecular genetics, DNA extraction protocols and kits are sample-specific and proprietary, preventing lateral distribution among similar facilities from different sectors to alleviate supply shortages during a crisis. Expanding upon previous fast extraction protocols such as alkaline- and detergent-based ones, the use of boiling-hot water to rupture cells, virions, and nuclei, as proposed during the COVID-19 pandemic, might alleviate shortages and costs. Different soft, relatively abundant (highly enriched), and uncomplicated (genomically homogenous and with few inhibitors) biosamples are collected in 1.

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The analysis of past epidemics and pandemics, either spontaneous or of human origin, may revise the physical history of microbiota and create a temporal context in our understanding regarding pathogen attributes like virulence, evolution, transmission and disease dynamics. The data of high-tech scientific methods seem reliable, but their interpretation may still be biased when tackling events of the distant past. Such endeavors should be adjusted to other cognitive resources including historical accounts reporting the events of interest and references in alien medical cultures and terminologies; the latter may contextualize them differently from current practices.

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