A combination of two electrophoretical approaches for detailed proteome-based characterization of SCLC subtypes.

Context: Small cell lung cancers (SCLC) are heterogeneous and tumours differ in growth characteristics and treatment resistance.

Objective: To get insight into the underlying protein profiles responsible for this heterogeneity, two subtypes of SCLC cells mutually differing in chemo resistance properties and growth characteristics are analysed.

Materials And Methods: Two different electrophoresis approaches in combination with mass spectrometry were used to detect differences between the SCLC cell lines GLC1 and GLC1M13: IEF/SDS-PAGE as well as cetyltrimethylammonium bromide (CTAB)-SDS-PAGE.