Publications by authors named "A V Sukhozhenko"
Enantiomers of 3-hydroxybutyric acid (3-HB) can be used as the chiral precursors for the production of various optically active fine chemicals, including drugs, perfumes, and pheromones. In this study, Escherichia coli was engineered to produce (S)-3-HB from glucose through the inverted reactions of the native aerobic fatty acid β-oxidation pathway. Expression of only specific genes encoding enzymes responsible for the conversion of acetyl-CoA to acetoacetyl-CoA, reduction of acetoacetyl-CoA to 3-hydroxybutyryl-CoA and subsequent hydrolysis of 3-hydroxybutyryl-CoA to 3-HB was directly upregulated in an engineered strain.
View Article and Find Full Text PDFCell surface display of Yarrowia lipolytica lipase Lip2p using the cell wall protein YlPir1p, its characterization, and application as a whole-cell biocatalyst.
Appl Biochem Biotechnol
April 2015
Article Synopsis
- The Yarrowia lipolytica lipase Lip2p was successfully displayed on yeast cells using a fusion with the YlPir1p protein, achieving a high hydrolytic activity of 11,900 U/g of dry weight.
- However, there was some leakage of the enzyme from the cell wall, with around 600,000 molecules of the recombinant enzyme present per cell, approaching the maximum limit.
- Despite the leakage, the surface-displayed lipase showed improved stability at high temperatures and in organic solvents, and was effectively utilized in recycling biodiesel synthesis, achieving significant yields of methyl esters in multiple reaction cycles.
Anaerobic biosynthesis of 1-butanol from glucose is investigated in recombinant Escherichia coli strains which form butyryl-CoA using the heterologous enzyme complex of clostridia or as a result of a reversal in the action of native enzymes of the fatty acid beta-oxidation pathway. It was revealed that when the basic pathways of acetic and lactic acid formation are inactivated due to deletions in the ackA, pta, poxB, and ldhA genes, the efficiency of butyryl-CoA biosynthesis and its reduced product, i.e.
View Article and Find Full Text PDFThe basic reactions of the clostridial 1-butanol biosynthesis pathway can be regarded to be the inverted reactions of the fatty acid β-oxidation pathway. A pathway for the biosynthesis of fuels and chemicals was recently engineered by combining enzymes from both aerobic and anaerobic fatty acid β-oxidation as well as enzymes from other metabolic pathways. In the current study, we demonstrate the inversion of the entire aerobic fatty acid β-oxidation cycle for 1-butanol biosynthesis.
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