Publications by authors named "A V Shutov"
Testosterone (TS) and its 1(2)-dehydrogenated derivative boldenone (BD) are widely used in medicine, veterinary science and as precursors in organic synthesis of many therapeutic steroids. Green production of these compounds is possible from androstenedione (AD) enzymatically, or from phytosterol (PS) using fermentation stages. In this study, the ascomycete sp.
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- Pregnenolone and progesterone are crucial steroid hormones in mammals, and new recombinant strains are needed for more efficient microbiological production from sterols.
- A new strain of Mycolicibacterium smegmatis was developed to express steroidogenic enzymes from the bovine adrenal cortex, leading to the production of progesterone and pregnenolone from cholesterol.
- The study demonstrated successful transformation of sterols under optimized conditions, yielding high-purity crystalline pregnenolone, which enhances the potential for efficient hormone production using microbial platforms.
The hydroxylation of steroids in the C7β position is one of the rare reactions that allow the production of value-added precursors in the synthesis of ursodeoxycholic acid and other pharmaceuticals. Recently, we discovered this activity in the ascomycete sp. VKM F-3040.
View Article and Find Full Text PDFMicrobial 1(2)-dehydrogenation of 3-ketosteroids is an important basis for the production of many steroid pharmaceuticals and synthons. When using the wild-type strains for whole cell catalysis, the undesirable reduction of the 20-carbonyl group, or 1(2)-hydrogenation, was observed. In this work, the recombinant strains of and were constructed with blocked endogenous activity of 3-ketosteroid-9α-hydroxylase, 3-ketosteroid-1(2)-dehydrogenase (3-KSD), and expressing 3-KSD encoded by the gene () from VKM Ac-2033D.
View Article and Find Full Text PDFExpression of Synthetic cyp102A1-LG23 Gene and Functional Analysis of Recombinant Cytochrome P450 BM3-LG23 in the Actinobacterium Mycolicibacterium smegmatis.
Biochemistry (Mosc)
September 2023
Article Synopsis
- Cytochrome CYP102A1 (P450 BM3) from Priestia megaterium is a promising candidate for directed evolution due to its unique functions and ability to hydroxylate androstanes into bioactive products.
- The study focused on expressing a mutant form of this enzyme, CYP102A1-LG23, in Mycolicibacterium smegmatis BD, achieving successful conversion of androst-4-ene-3,17-dione into 7β-OH-AD.
- Co-expressing the mutant enzyme with glucose dehydrogenase (GDH) led to a significant increase in hydroxylation yield, demonstrating the potential for engineered bacteria to produce valuable steroid compounds.