Human immunodeficiency virus type 1 integrase is one of three viral enzymes, and it realizes a key process of the viral replication cycle, i.e. viral DNA integration into infected cell genome.
View Article and Find Full Text PDFReaction of PtII(DAPO)X2 complexes (where DAPO is trans-3,4-diamino-2,2,6,6-tetramethylpiperidine-1-oxyl, X2 = (NO3)2, oxalato (Ox) or 1,1-cyclobutanedicarboxylato (Cbdca)) with a bovine spleen DNA in 0.01 M NaHCO3 at 37 degrees C for 24 h gives rise to formation of platinated DNA. The [bound PtII(DAPO)]/[nucleotide] ratio (r) depends on the initial ratio of the reagents and on the nature of leaving ligands X.
View Article and Find Full Text PDFAt cot around 20, the rat liver DNA reassociation curves were obtained 12-48 hours after injection of the animals with sublethal doses of cycloheximide. In total preparations of DNA-24 and DNA-36 at cot 0.02-0.
View Article and Find Full Text PDFA chromatin preparation isolated one hour after cycloheximide injection possesses a higher RNA-synthesizing activity and is split by DNAase I to a greater extent as compared to chromatin of control hepatocytes. Thermal denaturation of this preparation causes a considerable increase (about 2-fold) in the content of the thermolabile fraction melting in the free DNA denaturation zone. The relative content of fast repetitions in the thermolabile fraction (1 hour after the beginning of experiment) is lower than that in control.
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