Publications by authors named "A V Shchulkin"
Article Synopsis
- The study investigated how sex hormones regulate the transporter protein OATP1B3 in HepG2 cells.
- Estradiol increased OATP1B3 levels significantly at 10 and 100 μM via the farnesoid X-receptor.
- Testosterone also raised OATP1B3 expression at all tested concentrations through the liver X-receptor α (LXRα), while progesterone showed no effect.
Effect of succinic acid on the processes of myogenesis was investigated in the study with the cells of C2C12 line. In the concentration range 10-1000 µM, succinic acid stimulated the process of myogenic differentiation, increasing the levels of myogenesis factors MyoD (at all stages of myogenesis) and myogenin (at the stage of terminal differentiation). Presence of the succinate receptors SUCNR1 was revealed in the C2C12 cells using Western blotting, level of which decreased during myogenesis.
View Article and Find Full Text PDFArticle Synopsis
- A study tested the effects of ethylmethylhydroxypyridine succinate (EMHPS) on male ICR mice experiencing acute alcohol intoxication induced by a 40% ethanol injection.
- Mice were treated with either EMHPS or saline shortly after alcohol administration, and their behavior was evaluated at 3 and 24 hours post-treatment.
- Results showed that both intravenous and intramuscular EMHPS significantly mitigated negative effects of alcohol, with treated mice displaying behavior similar to the control group and demonstrating neuroprotective properties.
The free radical and cytokine statuses of the cornea during its thermal burn and the possibility of its correction by lactoferrin have been studied in Soviet Chinchilla rabbits. The development of a corneal thermal burn was accompanied by the development of oxidative stress (increased levels of TBA-reactive substances and carbonyl derivatives of proteins, decreased activity of SOD and GPx enzymes) and a pronounced inflammatory reaction with increased levels of TNF-1α, IL-10, TGF-1β. The use of lactoferrin had a pronounced therapeutic effect, which was manifested by accelerated healing, prevention of the development of complications (corneal perforations), a decrease in the severity of oxidative stress, an increase in the concentrations of TNF-1α (in the early stages), IL-10 (in the later stages), TGF-1β (throughout the experiment).
View Article and Find Full Text PDFArticle Synopsis
- - P-glycoprotein (Pgp) is an important protein that transports molecules out of cells, playing a key role in drug absorption, distribution, and resistance to chemotherapy.
- - The regulation of Pgp during oxidative stress is complex and involves transcription factors like Nrf2 and Nf-kB, which interact under various conditions influenced by the severity of the oxidative stress.
- - While oxidative stress typically increases Pgp expression, severe stress can actually reduce it due to amino acid oxidation; additionally, Pgp helps mitigate oxidative stress by eliminating harmful factors and participating in signaling pathways.