Organogenesis in a Broad Spectrum of Grape Genotypes and -Mediated Transformation of the Podarok Magaracha Grapevine Cultivar.

We present data on the ability for organogenesis in 22 genotypes of grapevine and developed a direct organogenesis protocol for the cultivar Podarok Magaracha and the rootstock Kober 5BB. The protocol does not require replacement of culture media and growth regulators, and the duration is 11 weeks. The cultivation of explants occurs on modified MS medium with the addition of 2.

CRISPR/Cas9-mediated мultiplexed multi-allelic mutagenesis of genes located on A, B and R subgenomes of hexaploid triticale.

The first-time generation of hexaploid triticale plants harbouring variable panels of novel mutations in gene families involved in starch biosynthesis has been achieved by the subgenome-independent multiplexed CRISPR/Cas9-mediated editing.

Endogenously Produced Jasmonates Affect Leaf Growth and Improve Osmotic Stress Tolerance in Emmer Wheat.

In light of recent climate change, with its rising temperatures and precipitation changes, we are facing the need to increase the valuable crop's tolerance against unfavorable environmental conditions. Emmer wheat is a cereal crop with high nutritional value. We investigated the possibility of improving the stress tolerance of emmer wheat by activating the synthesis of the stress hormone jasmonate by overexpressing two genes of the jasmonate biosynthetic pathway from , () and ().

Overexpression Compromises Tolerance to in Hexaploid and Tetraploid Wheat.

12-Oxophytodienoate reductase is the enzyme involved in the biosynthesis of phytohormone jasmonates, which are considered to be the major regulators of plant tolerance to biotic challenges, especially necrotrophic pathogens. However, we observe compromised tolerance to the necrotrophic fungal pathogen in transgenic hexaploid bread wheat and tetraploid emmer wheat plants overexpressing gene from , while in Arabidopsis plants themselves, endogenously produced and exogenously applied jasmonates exert a strong protective effect against . Exogenous application of methyl jasmonate on hexaploid and tetraploid wheat leaves suppresses tolerance to and induces the formation of chlorotic damages.