Publications by authors named "A V Poddubikov"

The indicators of innate immunity and the composition of the microbiome in the nasopharyngeal mucosa in centenarians with different aging phenotypes were analyzed. A significant increase in the expression of pattern-recognizing receptor genes (TLR2, TLR4, and NLRP3) and proinflammatory cytokines (IL1B, IL18) was shown in the group of centenarians with pathological aging phenotype. In centenarians with successful aging phenotype, increased diversity of the microbiome composition was observed.

View Article and Find Full Text PDF

The species identity of the studied lactobacillus strains was confirmed by matrix-activated laser desorption/ionization with time-of-flight ion separation (MALDI-TOF mass spectrometry). Lactobacillus strains differed in the dynamics of lactic acid accumulation and changes in the pH of the culture medium. The culture medium affected adhesion ability of lactobacilli.

View Article and Find Full Text PDF
Article Synopsis
  • The study investigates how one or two doses of inactivated adjuvanted influenza vaccines affect immune responses, specifically focusing on the expression of endosomal Toll-like receptors (TLRs) in patients with common variable immunodeficiency (CVID).
  • Six CVID patients received one vaccine dose followed by nine patients who received two doses; flow cytometry was used to analyze immune cell populations and TLR expression.
  • Findings show that while key lymphocyte populations remained stable post-vaccination, patients receiving two doses exhibited increased TLR3 and TLR9 expression, indicating that two-dose vaccine regimens may enhance immune responses in CVID patients.
View Article and Find Full Text PDF

We studied toxicity of recombinant Streptococcus pneumoniae pneumolysin protein in experiments on mice and its cytopathogenic effect on cultures of Vero green monkey kidney cells and human lung carcinoma A549 cells in vitro. In vivo and in vitro experiments proved the absence of compromised toxicity and direct cytopathogenic action of the recombinant protein.

View Article and Find Full Text PDF

Pneumolysin (Ply) is a target for the development of serotype-independent pneumococcal vaccines, an important condition for the efficacy of which is their ability to activate innate immunity with the subsequent formation of adaptive immunity. In this study, the ability of recombinant full-length Ply (rPly) of pneumococci to induce TLR expression and maturation of dendritic cells generated from mouse bone marrow was evaluated. It was shown that rPly in vitro increased the number of dendritic cells expressing Toll-like receptor 4 (TLR4) on the membrane.

View Article and Find Full Text PDF