[RNA ligase from bacteriophage T4. VIII. Solid phase enzymatic synthesis of oligoribonucleotides].

A method of the solid-phase enzymic synthesis of oligoribonucleotides has been suggested. The donor is fixed through its 3'-end on a water-insoluble matrix followed by the stepwise RNA ligase- and T4 polynucleotide kinase-assisted coupling of trinucleoside diphosphates in the 5'-direction. As an example, (pA)6pAox was immobilised on Biogel P-300 hydrazine and the RNA ligase-catalyzed addition of acceptor ApApA to the donor gave (Ap)9 with the 50% yield.

[Study of intralysosomal protein catabolism using lysosomotropic agents--proteolysis and protease inhibitors].

Inhibition of intralysosomal catabolism of proteins was studied in rat liver cells using lysosomotropic drugs suramin (single administration at a dose of 250 mg/kg within 24 and 48 hrs) and chloroquine (at a dose of 30 mg/100 g within 0.5, 1, 3, 6 and 12 hrs after administration). Suramine inhibited pure preparations of cathepsin B and L, while chloroquine inhibited cathepsins B, L and H.