Publications by authors named "A V Maslov"
Article Synopsis
- * Researchers used metagenomic and cultivation-based methods to analyze the microbiomes and found stable microbial communities similar to those from critical early Earth periods, indicating a link between present-day microbes and historical biospheric evolution.
- * The Upper Jurassic aquifer, rich in CO and influenced by magmatic processes, hosts a unique ecosystem that may reflect early Earth conditions, contributing valuable insights into microbial evolution and the formation of the modern biosphere.
At over 200 years, the maximum lifespan of the bowhead whale exceeds that of all other mammals. The bowhead is also the second-largest animal on Earth, reaching over 80,000 kg. Despite its very large number of cells and long lifespan, the bowhead is not highly cancer-prone, an incongruity termed Peto's Paradox.
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- The study aimed to analyze DNA methylation from bone samples, compare it with whole blood samples, and investigate its relationship with 1-year mortality in elderly patients with hip fractures.
- The researchers collected samples from 47 patients aged 65 and older, focusing on 12 subjects for detailed analysis, finding no significant difference in DNA yield between blood and bone.
- Results indicated that patients who did not survive had a significantly higher mean DNA methylation age derived from both bone and blood compared to those who survived, with a strong correlation (R = 0.81) between the two tissue types' methylation ages.
Thus far, multiple techniques for single cell analysis have been developed, yet we lack a relatively simple tool to assess DNA and RNA from the same cell at whole-transcriptome and whole-genome depths. Here we present an updated method for physical separation of cytoplasmic RNA from the nuclei, which allows for simultaneous studies of DNA and RNA from the same single cell. The method consists of three steps-(1) immobilization of a single cell on solid substrate, (2) hypotonic lysis of immobilized single cell, and (3) separation of cytosol containing aqueous phase and immobilized nucleus.
View Article and Find Full Text PDFLabel-free super-resolution (LFSR) imaging relies on light-scattering processes in nanoscale objects without a need for fluorescent (FL) staining required in super-resolved FL microscopy. The objectives of this Roadmap are to present a comprehensive vision of the developments, the state-of-the-art in this field, and to discuss the resolution boundaries and hurdles which need to be overcome to break the classical diffraction limit of the LFSR imaging. The scope of this Roadmap spans from the advanced interference detection techniques, where the diffraction-limited lateral resolution is combined with unsurpassed axial and temporal resolution, to techniques with true lateral super-resolution capability which are based on understanding resolution as an information science problem, on using novel structured illumination, near-field scanning, and nonlinear optics approaches, and on designing superlenses based on nanoplasmonics, metamaterials, transformation optics, and microsphere-assisted approaches.
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