[Budd-Chiari syndrome (case report)].

Budd-Chiari syndrome is a rare disease defined by the obstruction of hepatic venous outflow anywhere from the small hepatic veins to the junction of the inferior vena cava and the right atrium. This syndrome is uncommon in children. The article presents a case report of chronic refractory Budd-Chiari syndrome with a leading ascites in 16 years adolescent, which required surgical intervention--transjugular intrahepatic porta systemic shunt.

[Use of non-radioactive biotin-labelled probes for detecting hepatitis A virus].

The biotin-labeled DNA probes were constructed on the basis of the hybrid bacteriophage M13nip 9 single-stranded DNA containing the fragments of the hepatitis A viral cDNA. The probes were biotin treated by chemical modification of the DNA by the peraminating reagent or photochemically. The labeled DNA probes were used in molecular hybridization experiments with the nuclear acids fixed on the nitrocellulose filters.