Multiple molecular recognition mechanisms. Cytochrome P450--a case study.

Biomolecular recognition is complex. The balance between the different molecular properties that contribute to molecular recognition, such as shape, electrostatics, dynamics and entropy, varies from case to case. This, along with the extent of experimental characterization, influences the choice of appropriate computational approaches to study biomolecular interactions.

[Embolism of aorta bifurcation].

Results of treatment of 146 patients with embolism of aorta bifurcation have been analyzed and compared to that of extremity arteries. The severity of general condition of the patient, ischemic intoxication, postoperative complications are responsible for high death rate: postoperative mortality--53.7%, general--60.

A new approach to the study of protein-protein interaction by FTIR: complex formation between cytochrome P450BM-3 heme domain and FMN reductase domain.

We describe a new approach to the study of protein-protein interaction using Fourier transform infrared spectroscopy (FTIR). This approach is based on the combination of FTIR technique with both protein titration experiments and the principal component analysis (factor analysis) of the IR absorption spectra in the 1500-1800 cm(-1) region for the protein mixtures. We have applied this approach to the interaction of the heme domain with the FMN domain of bacterial monooxygenase cytochrome P450BM-3 (CYP102A1).

[Tumor necrosis factor, interleukin-6, endotoxin and procalcitonin in septic shock in patients with hematologic neoplasms].

Aim: To study changes in proinflammatory markers and mediators in septic shock in patients with hematologic malignancy (HM).

Material And Methods: The examination of 33 patients with HM and septic shock included measurement of plasma concentrations of tumor necrosis factor (TNF), interleukine-6 (IL-6), endotoxin, procalcitonin (PCT) 12-24 hours before and each 12 hours after shock; registration of central hemodynamics parameters, the condition severity by APACHE II.

Results: Out of 33 patients 18 died of refractory shock, 15 survived the shock.