The problems of functioning of health care system of the Kirghiz Republic and ways of their solving.

The article presents general analysis of national health care system of the Kirghiz Republic, including particular problems government agencies encounter, role of non-communicable diseases in increasing of total mortality, morbidity and disability of the population. The data is presented concerning economic losses associated with decreasing of labor productivity because of non-communicable diseases morbidity. The priority measures to strengthen primary health care organizations and ways of resolving existing problems in national health care are discussed.

The results of analysis of assessment of quality of medical care in the Kyrgyz Republic.

The article presents results of analysis of quality of medical care of population in the Kyrgyz Republic. The calculated index of quality of primary health care organizations and structure of defects at primary level of health care by region are considered. The analysis of examination of quality of medical services at in-patient level demonstrated annual increase of unjustified hospitalizations and detected defects in examination and treatment of patients.

[POSSIBILITY OF USING NESTED POLYMERASE CHAIN REACTION FOR DIAGNOS- TICS OF DISEASES CAUSED BY VARICELLA ZOSTER VIRUS].

Aim: Demonstrate the possibility of using nested PCR method for determination of Varicella Zoster virus (VZV) in clinical samples of peripheral blood of patients.

Materials And Methods: Material from 35 patients with clinical manifestations of herpes zoster and control group of 20 healthy donors was used in the study. Monocyte fraction of venous blood cells, pretreated with heparin, was isolated by centrifugation in ficoll-verografin density gradient, total DNA was then isolated from cells by phenol-chloroform extraction with subsequent precipitation with alcohol.

C-terminal interactors of the AMPA receptor auxiliary subunit Shisa9.

Shisa9 (initially named CKAMP44) has been identified as auxiliary subunit of the AMPA-type glutamate receptors and was shown to modulate its physiological properties. Shisa9 is a type-I transmembrane protein and contains a C-terminal PDZ domain that potentially interacts with cytosolic proteins. In this study, we performed a yeast two-hybrid screening that yielded eight PDZ domain-containing interactors of Shisa9, which were independently validated.