Publications by authors named "A V Karabelsky"
Cancer is one of the leading causes of death worldwide. Traditional cancer treatments include surgery, radiotherapy, and chemotherapy, as well as combinations of these treatments. Despite significant advances in these fields, the search for innovative ways to treat malignant tumors, including the application of oncolytic viruses, remains relevant.
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- Adeno-associated virus (AAV)-based gene therapy aims to treat hereditary disorders by replacing faulty genes, but the challenge is that many of these genes are larger than AAV's 5 kilobase capacity.
- Researchers are exploring strategies like truncated gene versions and intein-based protein trans splicing to restore full gene functionality, focusing on the effectiveness of different inteins from the DnaE group.
- The NpuDnaE variant has shown promising results, achieving 80% GFP assembly in HEK293 cells, indicating potential for higher efficiency in gene therapy applications.
Evaluation of mesenchymal stem cells as an model for inherited retinal diseases.
Front Cell Dev Biol
November 2024
Article Synopsis
- Retinal pathologies, particularly inherited retinal diseases (IRDs) like retinitis pigmentosa, are key contributors to vision impairment and blindness, necessitating effective research models for understanding and developing treatments.
- Various types of cells, including retina-specific cell lines, primary retinal cells, and mesenchymal stem cells (MSCs), can be utilized to create models of IRDs, with a focus on MSCs due to their effective differentiation into retinal cells.
- MSC-based models offer advantages over induced pluripotent stem cell (iPSC) models, being cheaper and quicker for drug screening purposes; however, the methods for differentiation still require optimization for better results.
Oncolytic Vesicular Stomatitis Virus: Optimisation Strategies for Anti-Cancer Therapies.
Front Biosci (Landmark Ed)
October 2024
Oncolytic viruses (OVs) represent a targeted anti-cancer therapy approach due to their ability not only to selectively infect and destroy malignant cells but also to induce an immune response. Vesicular stomatitis virus (VSV) offers a promising platform due to its low prevalence and pathogenicity in humans, lack of pre-existing immunity, easily manipulated genome, rapid growth to high titers in a broad range of cell lines, and inability to integrate into the host genome. However, despite its many advantages, many unresolved problems remain: problematic production based on the reverse genetics system, oncological selectivity, and the overall effectiveness of VSV monotherapy.
View Article and Find Full Text PDFMicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression by inhibiting the translation of target transcripts. The expression profiles of miRNAs vary in different tissues and change with the development of diseases, including cancer. This feature has begun to be used for the modification of oncolytic viruses (OVs) in order to increase their selectivity and efficacy.
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