Publications by authors named "A V Gussev"
Article Synopsis
- Alpha-fetoprotein (AFP) is a protein found in mammalian blood during embryonic development, consisting of a single glycosylated chain with multiple epitope clusters.
- Researchers developed three methods for separating variants of AFP based on different epitope expressions using technologies like immunoaffinity electrochromatography and electrophoresis/immunoblotting.
- The techniques allowed for the identification of hidden (cryptic) epitopes in AFP, which has important implications that are explored in the study's findings.
The method for comparison of epitope specificity of different monoclonal antibodies to one antigen, using a panel of monoclonal antibodies to mouse and human alpha-fetoprotein is described. The method used exploits the special properties of electroendosmotic flow in nitrocellulose membranes under the conditions of anionic isotachophoresis. Electroendosmosis allows successive transfer of several immunoreagents to the dots of monoclonals previously bound to the nitrocellulose membrane.
View Article and Find Full Text PDFA metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant one (multidrug resistance). Tetradomas were selected in the presence of two selective agents: aminopterin and actinomycin D. Using this approach we produced tetradomas secreting bispecific MAbs to horseradish peroxidase and human alpha-fetoprotein.
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