[Morphofunctional characterization and DNA stability of spermatozoa in primary examination of men fertility].

Aim: To assess the morphological and functional characteristics of spermatozoa and DNA stability in patients with normal seed parameters and with an ITZ exceeding the threshold value.

Patients And Methods: 85 males were studied for semen quality parameters. The morphological anomalies of head, middle piece, and tail of spermatozoa were estimated and presented in illustrations.

Uninephrectomized High-Fat-Fed Nicotinamide-Streptozotocin-Induced Diabetic Rats: A Model for the Investigation of Diabetic Nephropathy in Type 2 Diabetes.

Type 2 diabetes (DM2) could be reproduced in rats with alimentary obesity by using low doses of streptozotocin (LD-STZ) as well as STZ in high doses with preliminary nicotinamide (NA) administration. However, STZ could induce tubulotoxicity. .

System of KISS-KISS1R: focus on peripheral signaling in androgen-dependent tissues in the experimentally induced model hypogonadotropic hypogonadism.

Unlabelled: Kisspeptins, ligands of G protein-coupled receptor 54 (GPR54) encoded by the KiSS-1 gene, have recently emerged as key gatekeepers of the gonadotropic axis. Unlike its role at the hypothalamus on GnRH secretion, the effects of kisspeptins on gonadal and other peripheral tissues need to be clarified.

The Purpose: To investigate the impact of experimentally induced hypogonadism in male rats on kisspeptins signaling in androgen-dependent tissues and blood.

[The effect of metformin on myocardial tolerance to ischemia in rats with diabetes mellitus type 2].

The effect of metformin on myocardial sensitivity to ischemia in rats with neonatal streptozotocin T2DM was investigated using the model of global ischemia-reperfusion in the isolated perfused heart. Metformin administration had no effect on infarct size. At the same time, infarct size in T2DM was significantly lower than in controls, which is indicative of the phenomenon of metabolic preconditioning in T2DM.

[The obtaining and analysis of physiological activity of secreted proteins of Noggin family].

We have developed methods for producing recombinant proteins of Noggin family (Noggin1 and Noggin2 of the Xenopus laevis frog) that can interact with BMP factors of TGF-beta superfamily. The genetic constructs which allow one to effectively obtain Noggin1 and Noggin2 from synthetic mRNA microinjected into Xenopus laevis early embryos, as well as in the prokaryotic expression system, were generated. The obtained proteins contain three Myc-tag epitopes on their N-terminus.