[2'fluoro derivatives of nucleosides as substrates of viral replicative nucleotide polymerases].

Substrate specificities of three viral replicative polymerases of different origins (HIV reverse transcriptase, hepatitis C virus RNA polymerase, and herpes virus DNA polymerase) towards 2'F-NTP were studied. Activated DNA, polyA-oligoUs and (2'F-A)20-oligoU6-complexes were used as templates. It was shown that all DNA polymerases studied can incorporate 2'F-NMP into the 3'-end of primer-template complexes.

[Forecasting heat and functional state of human exposed to cooling in water medium].

Based on mathematic and statistic analysis of results obtained in studies of human heat exchange with cooling water medium, the authors represented canonical correlational patterns to determine integral parameter of cooling conditions (IPCC) referred to naked human and with various clothes on, both with and without additional heat releasing sources. Mathematic and statistic analysis helped to present correlational patterns for predicting levels of changes in human functional state according to IPCC comprising complex of factors that determine heat exchange in water medium, including safe time for stay in it.

[The performance quality of a flight trainer operator under conditions of heat discomfort].

The studies were conducted on 5 sound operators, age 26-44. The flight simulator was placed into a heat chamber with temperature range from 25 degrees C to 60 degrees C. In these conditions the operators had to withstand pitching and rolling in "horizontal flight" within 6 min.

[Secretion of recombinant human epidermal growth factor into the periplasm in Escherichia coli cells].

Secretion vectors were constructed in which a synthetic gene of human epidermal growth factor (hEGF) joined with a gene coding for the leader peptide to one of the E. coli outer membrane major proteins (OmpF) is controlled by tac promoter. The increase of the hEGF yield was achieved by the multiplication of the gene copies.

[Expression of a synthetic gene for human interleukin-4 in E. coli cells. Preparation of a biologically active protein].

Expression E. coli plasmid were constructed in which the human interleukin-4 (hIL4) synthetic gene is controlled by tac promoter. The expression level of the gene depends on the distance between RBS and the initial codon ATG, with the maximal production in case of the nine base pair distance.