[The developoment of total complement activity assay based on complement-dependent immune liposome lysis].

Background: The purpose of work was development of a fast and reproduced procedure for measurement of the total complement activity (TCA) in human or animal blood serum.

Materials And Methods: Steady at storage liposomes preparations, which surface sensitized 2,4-DNP haptens, and the internal volume contains calceine or sulforhodamine 101 are obtained. Complement-dependent immune lysis of liposomes at presence of the anti-2,4-DNP immunoglobulines and complement preparations from animals are investigated.

[A method of preparing a reagent for assessing the activity of the second component and factor B of the complement system].

The method is intended for preparing a reagent for the detection of the second component and factor B of the complement system. It makes use of an unsophisticated device for even time-controlled heating of the serum by letting it pass through a coil pipe submerged in the bath. The resultant reagent is used to measure the activity of the second component and B factor of the complement system and may be used for assessing humoral immunity in patients.

[Rapid method of measuring the alternative activity of the complement system].

The method is based on the ability of inulin to activate the alternative route of the complement system. The alternative activity is assessed from the consumption of the third complement component as a result of interaction with inulin. C3 is measured in experimental and control samples by kinetic titration, which permits assessing the activation of the alternative route in patients sera in less than 2 hours.