Monitoring of brain spiking activity and autoantibodies to N-terminus domain of GluR1 subunit of AMPA receptors in blood serum of rats with cobalt-induced epilepsy.

We have monitored EEG spontaneous spiking activity and analyzed serum from rats with cobalt-induced epilepsy for the presence of autoreactive antibodies to alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) glutamate receptor subunits. The presence and the level of autoantibodies were assessed using immunoblot and ELISA with synthetic peptide specific to the N-terminus domain of the GluR1 subunit of the AMPA receptor. Rats with cobalt-induced epilepsy exhibited strong GluR1 immunoreactivity at the end of the first week after surgery compared with vehicle-treated rats.

Spike morphology in PTZ-induced generalized and cobalt-induced partial experimental epilepsy.

Interictal epileptiform EEG activity was recorded in 10 rats with pentylenetetrazol-induced generalized seizures and in 10 rats with cobalt-induced partial epilepsy. Thirty spikes were averaged for each rat, and morphological parameters of the average waveform (such as amplitude, duration, and 1st derivative of the rising and falling spike deflections, and sharpness at peak) were measured. Pentylenetetrazol rats showed significantly higher and longer spikes than cobalt animals with a faster 1st derivative of both deflections; sharpness at peak was also higher in this group.